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Sample GSM4079388 Query DataSets for GSM4079388
Status Public on Jun 22, 2021
Title TRAP 7-10h IP S59 replicate3
Sample type RNA
 
Source name Drosophila embryos from stage 7-10h
Organism Drosophila melanogaster
Characteristics origin: Staged collection
age: 7-10h AEL
muscle population: S59
Growth protocol Embryos are collected from Drosophila fly population cages grown at 25 degres for 3 hours. Plates are then collected and kept at 25 degres for 7, 10 or 13 more hours to reach desired time windows
Extracted molecule total RNA
Extraction protocol After collection, embryos are washed and lysed in order to extract and immunoprecipitate GFP-tagged polysomes and associated RNA. Trizol extraction is then performed to purify RNA
Label CY3
Label protocol Labelling (Low Input Quick Amp WT Labeling Kit, One-Color), hybridization and scan were done at the Genecore genomics facility at EMBL Heidelberg using agilent 8X60K arrays technology (Agilent p/n G2534-60014) starting with 10ng of purified RNA.
 
Hybridization protocol Agilent Feature Extraction Software (v 10.7.3) was used for background subtraction and LOWESS normalization.
Scan protocol Standard Agilent protocol
Data processing signal intensities were normalized using quantile-quantile normalization with solo2 software. Log2 fold change were computed and p-value computed using t-test
 
Submission date Sep 13, 2019
Last update date Jun 22, 2021
Contact name yoan renaud
E-mail(s) yoan.renaud@uca.fr
Organization name igred
Street address place henri dunand
City clermont ferrand
ZIP/Postal code 63000
Country France
 
Platform ID GPL27460
Series (1)
GSE137443 TRAP analysis of somatic muscle populations in Drosophila embryos

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
1 216.379
2 5.20953
3 5.18456
4 79.2991
5 5.35662
7 5.08986
8 5.06312
9 29.8745
11 134.444
12 19.2962
13 6.59836
14 7.37246
15 4.93758
16 6.53395
17 4.82033
18 400.49
19 4.77479
21 15.4503
23 232.334
24 4.66627

Total number of rows: 44921

Table truncated, full table size 601 Kbytes.




Supplementary file Size Download File type/resource
GSM4079388_S59_7-10_IP_3.txt.gz 8.6 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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