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Status |
Public on Jun 22, 2021 |
Title |
TRAP 7-10h IP S59 replicate3 |
Sample type |
RNA |
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Source name |
Drosophila embryos from stage 7-10h
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Organism |
Drosophila melanogaster |
Characteristics |
origin: Staged collection age: 7-10h AEL muscle population: S59
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Growth protocol |
Embryos are collected from Drosophila fly population cages grown at 25 degres for 3 hours. Plates are then collected and kept at 25 degres for 7, 10 or 13 more hours to reach desired time windows
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Extracted molecule |
total RNA |
Extraction protocol |
After collection, embryos are washed and lysed in order to extract and immunoprecipitate GFP-tagged polysomes and associated RNA. Trizol extraction is then performed to purify RNA
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Label |
CY3
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Label protocol |
Labelling (Low Input Quick Amp WT Labeling Kit, One-Color), hybridization and scan were done at the Genecore genomics facility at EMBL Heidelberg using agilent 8X60K arrays technology (Agilent p/n G2534-60014) starting with 10ng of purified RNA.
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Hybridization protocol |
Agilent Feature Extraction Software (v 10.7.3) was used for background subtraction and LOWESS normalization.
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Scan protocol |
Standard Agilent protocol
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Data processing |
signal intensities were normalized using quantile-quantile normalization with solo2 software. Log2 fold change were computed and p-value computed using t-test
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Submission date |
Sep 13, 2019 |
Last update date |
Jun 22, 2021 |
Contact name |
yoan renaud |
E-mail(s) |
yoan.renaud@uca.fr
|
Organization name |
igred
|
Street address |
place henri dunand
|
City |
clermont ferrand |
ZIP/Postal code |
63000 |
Country |
France |
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|
Platform ID |
GPL27460 |
Series (1) |
GSE137443 |
TRAP analysis of somatic muscle populations in Drosophila embryos |
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