|
Status |
Public on May 27, 2010 |
Title |
Control tumor9 |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
Squamous Cell Carcinoma
|
Organism |
Mus musculus |
Characteristics |
tumor type: SCC tissue: Skin
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA extracted using Dneasy Kit following manufacturer's instructions
|
Label |
Cy3
|
Label protocol |
Agilent Oligonucleotide Array-Based CGH for Genomic DNA Analysis Protocol Version 5.0 was followed to label 1 µg of experimental or reference DNA
|
|
|
Channel 2 |
Source name |
C57BL/6 reference genomic DNA
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6
|
Biomaterial provider |
Jackson Laboratory
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA extracted using Dneasy Kit following manufacturer's instructions
|
Label |
Cy5
|
Label protocol |
Agilent Oligonucleotide Array-Based CGH for Genomic DNA Analysis Protocol Version 5.0 was followed to label 1 µg of experimental or reference DNA
|
|
|
|
Hybridization protocol |
labelled samples were prepared for hybridization using the Agilent aCGH Hybridization Kit and hybridized to arrays using Agilent Hybridization Oven (G2545A).
|
Scan protocol |
Washed and dryed slides were scanned on an Agilent G2565BA scanner. Images were quantified using Agilent Feature Extraction Software (version 9.5.3.1).
|
Description |
Control group
|
Data processing |
The Agilent Feature Extraction Software (v 9.5.31) was used for background subtraction and LOWESS normalization.
|
|
|
Submission date |
May 29, 2009 |
Last update date |
May 29, 2009 |
Contact name |
Enrique Torchia |
E-mail(s) |
enrique.torchia@ucdenver.edu
|
Organization name |
University of Denver
|
Department |
Dermatology
|
Street address |
12800 E 19th Ave, Rm P18-8123
|
City |
Aurora |
State/province |
CO |
ZIP/Postal code |
80045 |
Country |
USA |
|
|
Platform ID |
GPL4092 |
Series (1) |
GSE16342 |
CGH Analysis of Murine Squamous Cell Carcinoma: Control vs. Tumors overexpressing Aurora Kinase A |
|