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Sample GSM410715 Query DataSets for GSM410715
Status Public on Jun 02, 2009
Title SOD1G93A triceps-7w, biol rep1
Sample type RNA
 
Source name 7week-old triceps from SODG93A mice
Organism Mus musculus
Characteristics strain: C57BL/6
gender: female mice
genetic modification: transgenic humanSODG93A
tissue: 7 week-old triceps
Treatment protocol Mice were sacrificed according to national and international laws on animal care; gastrocenemius and triceps muscles were removed and immediately frozen in liquid nitrogen.
Growth protocol Mice were maintained at 21C with relative humidity 55+10% and 12hrs of light; Food (standard pellet) and water were supplied ad libitum.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with Trizol reagent and subsequently purified on Qiagen RNeasy mini-columns; quality was assessed on a BioAnalyzer instrument (Agilent).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA.
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on murine 430A2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description Gene expression data from presymptomatic SODG93A triceps
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
 
Submission date Jun 01, 2009
Last update date Jun 01, 2009
Contact name Antonia Ratti
E-mail(s) antonia.ratti@unimi.it
Organization name IRCCS Istituto Auxologico Italiano, University of Milan
Department Dept. Neurological Sciences
Lab Lab. Neuroscience
Street address Via Zucchi, 18
City Cusano Milanino
State/province Milan
ZIP/Postal code 20095
Country Italy
 
Platform ID GPL8321
Series (2)
GSE16360 Gene expression profiling of muscles from transgenic humanSODG93A mice at presymptomatic stage
GSE16362 ALS mice muscle gene expression data

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1415670_at 6.667416818 P 0.001672877
1415671_at 7.527489379 P 0.001672877
1415672_at 8.250646395 P 0.003822926
1415674_a_at 7.28963683 P 0.00221961
1415675_at 6.313726208 P 0.006396093
1415676_a_at 9.927146505 P 0.001672877
1415678_at 9.764773718 P 0.001672877
1415679_at 9.548633502 P 0.001672877
1415681_at 7.756747596 P 0.002923594
1415682_at 7.15992048 P 0.001672877
1415683_at 7.801586296 P 0.001672877
1415684_at 6.120498605 P 0.001672877
1415685_at 7.007093622 P 0.001672877
1415686_at 7.825856742 P 0.00221961
1415687_a_at 10.24114716 P 0.001672877
1415688_at 9.206935675 P 0.001672877
1415689_s_at 5.969869698 P 0.020429855
1415690_at 8.755234414 P 0.00221961
1415691_at 8.672468448 P 0.001672877
1415692_s_at 8.409803641 P 0.001672877

Total number of rows: 7729

Table truncated, full table size 283 Kbytes.




Supplementary file Size Download File type/resource
GSM410715.CEL.gz 1.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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