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Status |
Public on Jun 02, 2009 |
Title |
SOD1G93A triceps-7w, biol rep1 |
Sample type |
RNA |
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Source name |
7week-old triceps from SODG93A mice
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Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 gender: female mice genetic modification: transgenic humanSODG93A tissue: 7 week-old triceps
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Treatment protocol |
Mice were sacrificed according to national and international laws on animal care; gastrocenemius and triceps muscles were removed and immediately frozen in liquid nitrogen.
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Growth protocol |
Mice were maintained at 21C with relative humidity 55+10% and 12hrs of light; Food (standard pellet) and water were supplied ad libitum.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted with Trizol reagent and subsequently purified on Qiagen RNeasy mini-columns; quality was assessed on a BioAnalyzer instrument (Agilent).
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Label |
biotin
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Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA.
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Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on murine 430A2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
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Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
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Description |
Gene expression data from presymptomatic SODG93A triceps
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Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
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Submission date |
Jun 01, 2009 |
Last update date |
Jun 01, 2009 |
Contact name |
Antonia Ratti |
E-mail(s) |
antonia.ratti@unimi.it
|
Organization name |
IRCCS Istituto Auxologico Italiano, University of Milan
|
Department |
Dept. Neurological Sciences
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Lab |
Lab. Neuroscience
|
Street address |
Via Zucchi, 18
|
City |
Cusano Milanino |
State/province |
Milan |
ZIP/Postal code |
20095 |
Country |
Italy |
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Platform ID |
GPL8321 |
Series (2) |
GSE16360 |
Gene expression profiling of muscles from transgenic humanSODG93A mice at presymptomatic stage |
GSE16362 |
ALS mice muscle gene expression data |
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