SREK: SREK was performed according to the manufacturer’s protocol (Ambion, Inc, Austin, TX). Two replicates were produced with the 15-40 nt fraction derived from 0.5 μg small RNA (<200 nt) as input material.
Library strategy
RNA-Seq
Library source
transcriptomic
Library selection
size fractionation
Instrument model
AB SOLiD System 2.0
Description
none
Data processing
Sequencing reads were mapped to the known miRNA and miRNA* species in miRBase v11.0 using SHRiMP. Reads with 3 or less colorspace errors were included in the further analysis.
