|
| Status |
Public on Mar 22, 2020 |
| Title |
Malignant mesothelioma 8 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
F1 hybrid rat non-neoplastic kidney
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: non-neoplastic kidney
strain: F1 hybrid (Brown-Norway X Fischer344)
Sex: male
|
| Treatment protocol |
F1 hybrid rats were injected with tremolite (5 mg) twice at 6th and 7th weeks. From 10th to 21st weeks, rats were treated with either saline or nitrilotriacetate (80 mg/kg body weight) once a week for 12 weeks. The rats were euthanized when they show the signs of MM developments.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using DNeasy Blood & Tissue Kit (QIAGEN).
|
| Label |
Cy3
|
| Label protocol |
The extracted genomic DNA was labeled using Aagilent Sure tag DNA labeling kit according to the manufacturer's protocol (Version 7.1).
|
| |
|
| Channel 2 |
| Source name |
MM_120-2
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: Malignant mesothelioma
strain: F1 hybrid (Brown-Norway X Fischer344)
Sex: male
treatment: Tremolite 10 mg
|
| Treatment protocol |
F1 hybrid rats were injected with tremolite (5 mg) twice at 6th and 7th weeks. From 10th to 21st weeks, rats were treated with either saline or nitrilotriacetate (80 mg/kg body weight) once a week for 12 weeks. The rats were euthanized when they show the signs of MM developments.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using DNeasy Blood & Tissue Kit (QIAGEN).
|
| Label |
Cy5
|
| Label protocol |
The extracted genomic DNA was labeled using Aagilent Sure tag DNA labeling kit according to the manufacturer's protocol (Version 7.1).
|
| |
|
| |
| Hybridization protocol |
The labeled DNA was hybridized with Agilent SurePrint G3 Rat CGH 4x180k microarray at 65°C for 24 hours according to the manufacturer's protocol (Version 7.1).
|
| Scan protocol |
The slides were scanned in an Agilent DNA microarray scanner with SureScan High-Resolution Technology (G2565CA).
|
| Data processing |
The scanned images were analyzed with Agilent Feature Extraction Software 10.7 using default parameters (CGH_107_Sep09 protocol).
|
| |
|
| Submission date |
Oct 18, 2019 |
| Last update date |
Mar 24, 2020 |
| Contact name |
Shinya Toyokuni |
| E-mail(s) |
akatsuka@med.nagoya-u.ac.jp
|
| Organization name |
Nagoya University
|
| Department |
Pathology
|
| Street address |
65 Tsuruma-Cho, Showa-Ku
|
| City |
Nagoya |
| State/province |
Aichi |
| ZIP/Postal code |
466-8550 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10451 |
| Series (1) |
| GSE139106 |
Genomic profiles of tremolite-induced malignant peritoneal mesotheliomas developed in rats |
|
