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Sample GSM4133297 Query DataSets for GSM4133297
Status Public on Dec 31, 2020
Title ATACseq_rep1_U2OS
Sample type SRA
Source name Human bone osteosarcoma epithelial cells-U2OS
Organism Homo sapiens
Characteristics cell status: native cells
cell line: U2OS
Growth protocol U2OS cells and HEK293 cells were grown in DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin until 80% confluent. HepG2 cells were grown in EMEM with 10% FBS and 1% penicillin and streptomycin. K562 cells were grown in RPMI supplemented with 10% heat-inactivated FBS, 2 mM GlutaMax, and 1% penicillin and streptomycin.
Extracted molecule genomic DNA
Extraction protocol For ATAC-seq, cells (5 x 104) were incubated with DNase I (Worthington cat# LS002007) to remove DNA from dead cells, washed with PBS, resuspended in lysis buffer, and treated with transposase from Nextera DNA sample Prep Kit (Illumina, cat# FC-121-1030).
Libraries were made through adding adpators on purified tagmented DNA, followed by double-sided bead purification. Purified products were sequenced with PE 150 cycles on an Illumina Nextseq500 system
Library strategy ATAC-seq
Library source genomic
Library selection other
Instrument model Illumina NextSeq 500
Data processing ATAC-seq Reads aligned to hg19 using BWA. Properly aligned reads were filtered to exclude PCR duplicates and processed as previously described (PMC3959825). Read start positions were shifted towards the 3’ end by 4 bp in for reads aligning to plus strand and 5 bp towards the 5’ end for reads aligning to minus strand.
Genome_build: hg19
Supplementary_files_format_and_content: bigwig files for visualization on igv
Submission date Oct 21, 2019
Last update date Jan 01, 2021
Contact name Keith Joung
Organization name MGH
Street address 149 13th st
City Charlestown
State/province MA
ZIP/Postal code 02129
Country USA
Platform ID GPL18573
Series (1)
GSE139190 Chromatin status and transcriptomics of HEK293, U2OS, HepG2 and K562
BioSample SAMN13070674
SRA SRX7030826

Supplementary file Size Download File type/resource 380.6 Mb (ftp)(http) BW
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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