|
| Status |
Public on Dec 14, 2022 |
| Title |
Islet_Flox_Rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Flox_Islet
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl6
Sex: male
age: 12 weeks
genotype/variation: Flox
tissue: islet
|
| Treatment protocol |
islets were isolated bu collagenase digestion of pancreas in situ and purifoed by density centrifugation and then by hand picking.
|
| Growth protocol |
12 week old male flox and KO mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted by Trizol and then purified by Qiagen RNA prep kits along with DNAse digestion on column.
|
| Label |
biotin
|
| Label protocol |
Biotin labelled aRNA was fragmented and then hybridized
|
| |
|
| Hybridization protocol |
Following fragmentation 12mcg of aRNA was used fro hybridization. 16 hours at 45 °C with rotation at 60 rpm in the Affymetrix GeneChip® Hybridization Oven 640. The arrays were washed and stained with a streptavidin, R-phycoerythrin conjugate stain using the Affymetrix GeneChip® Fluidics Station 450. Signal amplification was done using biotinylated antistreptavidin.
|
| Scan protocol |
The stained arrays were scanned on the Affymetrix GeneChip® Scanner 3000. The images were analyzed and quality control metrics recorded using Affymetrix Command Console software version 3.0.0.
|
| Data processing |
affymetrix-algorithm-name = rma
affymetrix-algorithm-version = 1.0
program-name = Expression Console
program-version = 1.2.0.20
|
| |
|
| Submission date |
Oct 22, 2019 |
| Last update date |
Dec 14, 2022 |
| Contact name |
Vijay K. Yechoor |
| E-mail(s) |
yechoorv@pitt.edu
|
| Organization name |
University of Pittsburgh
|
| Lab |
Yechoor lab
|
| Street address |
200 Lothrop Street, BST 1058W
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE139228 |
Expression data from Tead1 beta cell KO |
|
