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Sample GSM4134368 Query DataSets for GSM4134368
Status Public on Dec 14, 2022
Title Islet_Tead1KO_Rep2
Sample type RNA
 
Source name Tead1KO_Islet
Organism Mus musculus
Characteristics strain: C57Bl6
Sex: male
age: 12 weeks
genotype/variation: Tead1 beta cell-specific KO
tissue: islet
Treatment protocol islets were isolated bu collagenase digestion of pancreas in situ and purifoed by density centrifugation and then by hand picking.
Growth protocol 12 week old male flox and KO mice
Extracted molecule total RNA
Extraction protocol total RNA was extracted by Trizol and then purified by Qiagen RNA prep kits along with DNAse digestion on column.
Label biotin
Label protocol Biotin labelled aRNA was fragmented and then hybridized
 
Hybridization protocol Following fragmentation 12mcg of aRNA was used fro hybridization. 16 hours at 45 °C with rotation at 60 rpm in the Affymetrix GeneChip® Hybridization Oven 640. The arrays were washed and stained with a streptavidin, R-phycoerythrin conjugate stain using the Affymetrix GeneChip® Fluidics Station 450. Signal amplification was done using biotinylated antistreptavidin.
Scan protocol The stained arrays were scanned on the Affymetrix GeneChip® Scanner 3000. The images were analyzed and quality control metrics recorded using Affymetrix Command Console software version 3.0.0.
Data processing affymetrix-algorithm-name = rma
affymetrix-algorithm-version = 1.0
program-name = Expression Console
program-version = 1.2.0.20
 
Submission date Oct 22, 2019
Last update date Dec 14, 2022
Contact name Vijay K. Yechoor
E-mail(s) yechoorv@pitt.edu
Organization name University of Pittsburgh
Lab Yechoor lab
Street address 200 Lothrop Street, BST 1058W
City Pittsburgh
State/province PA
ZIP/Postal code 15213
Country USA
 
Platform ID GPL1261
Series (1)
GSE139228 Expression data from Tead1 beta cell KO

Data table header descriptions
ID_REF
VALUE Quantification

Data table
ID_REF VALUE
AFFX-BioB-5_at 8.71106
AFFX-BioB-M_at 9.17089
AFFX-BioB-3_at 9.06174
AFFX-BioC-5_at 9.69574
AFFX-BioC-3_at 10.0322
AFFX-BioDn-5_at 11.1361
AFFX-BioDn-3_at 11.5232
AFFX-CreX-5_at 13.5697
AFFX-CreX-3_at 13.5817
AFFX-DapX-5_at 3.268
AFFX-DapX-M_at 3.20886
AFFX-DapX-3_at 3.43225
AFFX-LysX-5_at 3.21486
AFFX-LysX-M_at 3.4229
AFFX-LysX-3_at 3.17635
AFFX-PheX-5_at 3.45005
AFFX-PheX-M_at 3.38174
AFFX-PheX-3_at 4.38201
AFFX-ThrX-5_at 3.4747
AFFX-ThrX-M_at 3.4705

Total number of rows: 45101

Table truncated, full table size 850 Kbytes.




Supplementary file Size Download File type/resource
GSM4134368_KO-2_irs_VY_8224_MA2_31868.rma.chp.gz 310.9 Kb (ftp)(http) CHP
GSM4134368_irs_VY_8224_MA2_31868.cel.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data provided as supplementary file

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