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Sample GSM4147335 Query DataSets for GSM4147335
Status Public on Nov 01, 2022
Title 8_muMT
Sample type SRA
 
Source name Kidney
Organism Mus musculus
Characteristics genotype: KO
kidney pair: C
Treatment protocol Mice were infected with UPEC trans urethrealy twice 1 hour apart and culled 6 hours later.
Growth protocol Mice were housed under SPF conditions]
Extracted molecule total RNA
Extraction protocol Samples were stored in RNAlater until processing. Samples were homoginised in lysis buffer (Ambion) and RNA extracted with Purelink RNA mini kit (Ambion)
Libraries were constructed with Illumina TruSeq total Stranded RNA library prep kit
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing Demultiplex with Casava
Alignment with Hisat2
Genome_build: Mm10
 
Submission date Nov 01, 2019
Last update date Nov 01, 2022
Contact name John Robert Ferdinand
Organization name University of Cambridge
Street address Department of Medicine
City Cambridge
ZIP/Postal code CB2 0QH
Country United Kingdom
 
Platform ID GPL24247
Series (1)
GSE139848 Analysis of UTI on WT and muMT KO mice
Relations
BioSample SAMN13181466
SRA SRX7087494

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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