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Sample GSM4147345 Query DataSets for GSM4147345
Status Public on Nov 01, 2022
Title 10_muMT (F480Hi CD11blo Macrophages)
Sample type SRA
Source name Kidney
Organism Mus musculus
Characteristics genotype: KO
Treatment protocol Cells were stimulated with 100 ng/ml LPS in complete RPMI for 2hrs at 37C with 5% CO2 after which cells were sorted as F4/80 high CD11b low
Growth protocol Cell suspensions were prepaired fresh from murine kidneys
Extracted molecule total RNA
Extraction protocol Samples were homoginised in RLT+ (Qiagen) and RNA extracted with Rneasy plus micro kit (Qiagen)
Libraries were constructed with Takara pico input stranded
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
Description 10_muMT
Data processing Demultiplex with Casava
Alignment with Hisat2
Genome_build: Mm10
Submission date Nov 01, 2019
Last update date Nov 01, 2022
Contact name John Robert Ferdinand
Organization name University of Cambridge
Street address Department of Medicine
City Cambridge
ZIP/Postal code CB2 0QH
Country United Kingdom
Platform ID GPL24247
Series (1)
GSE139851 Analysis of LPS stimulation on F480Hi CD11blo Macrophages from WT and muMT KO mice
BioSample SAMN13181521
SRA SRX7087549

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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