|
Status |
Public on Nov 01, 2022 |
Title |
4_WT (F480Hi CD11blo Macrophages) |
Sample type |
SRA |
|
|
Source name |
Kidney
|
Organism |
Mus musculus |
Characteristics |
genotype: WT
|
Treatment protocol |
Cells were stimulated with 100 ng/ml LPS in complete RPMI for 2hrs at 37C with 5% CO2 after which cells were sorted as F4/80 high CD11b low
|
Growth protocol |
Cell suspensions were prepaired fresh from murine kidneys
|
Extracted molecule |
total RNA |
Extraction protocol |
Samples were homoginised in RLT+ (Qiagen) and RNA extracted with Rneasy plus micro kit (Qiagen) Libraries were constructed with Takara pico input stranded
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
4_WT
|
Data processing |
Demultiplex with Casava Alignment with Hisat2 Genome_build: Mm10
|
|
|
Submission date |
Nov 01, 2019 |
Last update date |
Nov 01, 2022 |
Contact name |
John Robert Ferdinand |
Organization name |
University of Cambridge
|
Street address |
Department of Medicine
|
City |
Cambridge |
ZIP/Postal code |
CB2 0QH |
Country |
United Kingdom |
|
|
Platform ID |
GPL24247 |
Series (1) |
GSE139851 |
Analysis of LPS stimulation on F480Hi CD11blo Macrophages from WT and muMT KO mice |
|
Relations |
BioSample |
SAMN13181519 |
SRA |
SRX7087551 |