|
| Status |
Public on Nov 05, 2019 |
| Title |
Ccrescentus_dcdnL_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Bacterial cells_dcdnL
|
| Organism |
Caulobacter vibrioides NA1000 |
| Characteristics |
genotype/variation: cdnL (CCNA_00690) deletion mutant
|
| Treatment protocol |
RNA extracted from 2 x 1ml OD660=0.3 for each replicate
|
| Growth protocol |
Biological triplicates of NA1000 vs NA1000-ΔcdnL grown in PYE at 28°C with agitation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol / Ambion RNA extraction kit
|
| Label |
Cy3
|
| Label protocol |
Roche / NimbleGen Cy3 labelling protocol
|
| |
|
| Hybridization protocol |
Roche / NimbleGen protocol
|
| Scan protocol |
Scanning with an Agilent microarray scanner at 3 μm resolution
|
| Data processing |
Normalization with Nimblescan implemented RMA algorithm over the whole chip.
|
| |
|
| Submission date |
Nov 04, 2019 |
| Last update date |
Nov 05, 2019 |
| Contact name |
Diego Gonzalez |
| Organization name |
University of Lausanne
|
| Department |
Department of Fundamental Microbiology
|
| Lab |
Prof. Justine Collier
|
| Street address |
Quartier UNIL-Sorge, Bâtiment Biophore
|
| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL17931 |
| Series (1) |
| GSE139873 |
Expression analysis of Caulobacter crescentus NA1000 bearing a CCNA_00690 (cdnL) deletion [dcdnL] vs Caulobacter crescentus NA1000 wild type [wt] |
|
