|
| Status |
Public on Nov 06, 2019 |
| Title |
ΔlytSR versus WT strain after 6h of culture replicat 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
cells
|
| Organism |
Staphylococcus lugdunensis |
| Characteristics |
strain: [delta]lytSR
time point: 6h of culture
|
| Growth protocol |
S. lugdunensis strains were grown in trypticase soy broth (TSB) at 37°C under shaking (150 rpm), and cells were collected at 6 and 8 h of growth.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini kit (Qiagen) following the manufacturer’s procedure. Two DNase treatments were successively performed using DNase I (Sigma-Aldrich) and Turbo DNA free (Thermo Fisher Scientific). Quality of RNA was evaluated using the RNA 6000 Nano with a Bioanalyzer (Agilent, Les Ulis, France).
|
| Label |
Cy5
|
| Label protocol |
cRNA was labelled with either Cy3 or Cy5
|
| |
|
| Channel 2 |
| Source name |
cells
|
| Organism |
Staphylococcus lugdunensis |
| Characteristics |
strain: Wild-Type
time point: 6h of culture
|
| Growth protocol |
S. lugdunensis strains were grown in trypticase soy broth (TSB) at 37°C under shaking (150 rpm), and cells were collected at 6 and 8 h of growth.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini kit (Qiagen) following the manufacturer’s procedure. Two DNase treatments were successively performed using DNase I (Sigma-Aldrich) and Turbo DNA free (Thermo Fisher Scientific). Quality of RNA was evaluated using the RNA 6000 Nano with a Bioanalyzer (Agilent, Les Ulis, France).
|
| Label |
Cy3
|
| Label protocol |
cRNA was labelled with either Cy3 or Cy5
|
| |
|
| |
| Hybridization protocol |
cRNA was co-hybridized on the microarray
|
| Scan protocol |
GE2_105_Dec08
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.7.3.1 (Agilent) using default parameters and data were extracted after Linear-Lowess normalisation. Genes with significant different expression (P0.05) were selected based on at least a 2 fold change in expression level.
|
| |
|
| Submission date |
Nov 04, 2019 |
| Last update date |
Nov 07, 2019 |
| Contact name |
jean-michel flaman |
| E-mail(s) |
jean-michel.flaman@lyon.unicancer.fr
|
| Phone |
+33687477812
|
| Organization name |
Inserm 1052
|
| Department |
CRCL
|
| Street address |
28 rue Laennec
|
| City |
Lyon |
| ZIP/Postal code |
69373 |
| Country |
France |
| |
|
| Platform ID |
GPL27718 |
| Series (1) |
| GSE139909 |
Role of the LytSR two-component regulatory system in Staphylococcus lugdunensis biofilm formation and pathogenesis |
|
