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Sample GSM4159970 Query DataSets for GSM4159970
Status Public on Feb 25, 2021
Title WT_Inhibitor_ChIP_2
Sample type SRA
 
Source name murine BM
Organism Mus musculus
Characteristics genotype: Smc3WT
inhibitor or dmso: Inhibitor
chip antibody: H3K27me3
Treatment protocol During passaing, cells were treated with 100uM DOT1L inhibitor (EPZ-5676) or vehicle (DMSO).
Growth protocol Bone marrow was isolated from Smc3+/f or WT mice that were treated with pIpC to induced excision of one Smc3 allele. Cells were collected after one week in passage.
Extracted molecule genomic DNA
Extraction protocol H3K27me3 antibody used: Millipore-Sigma 07-449. ChIP DNA was isolated by phenol-chloroform isolation.
ChIP libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit.
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina NextSeq 500
 
Description H3K27me3 ChIP
Data processing Reads were aligned with Bowtie2 to mm10, using default parameters on basepairtech.com
Peaks were called using MACS2, using default parameters on basepairtech.com
Genome_build: mm10
Supplementary_files_format_and_content: bigwig files
 
Submission date Nov 13, 2019
Last update date Feb 25, 2021
Contact name Katelyn Elizabeth Heimbruch
Organization name Medical College of Wisconsin
Street address 8727 Watertown Plank Road
City Miwaukee
State/province Wisconsin
ZIP/Postal code 53213
Country USA
 
Platform ID GPL19057
Series (2)
GSE140360 DOT1L inhibitors block abnormal self-renewal induced by cohesin loss [ChIP-seq]
GSE140361 DOT1L inhibitors block abnormal self-renewal induced by cohesin loss
Relations
BioSample SAMN13280798
SRA SRX7135938

Supplementary file Size Download File type/resource
GSM4159970_WTI2_27.bigwig 116.3 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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