|
| Status |
Public on Sep 30, 2020 |
| Title |
B_d2_1 |
| Sample type |
SRA |
| |
|
| Source name |
MEF
|
| Organism |
Mus musculus |
| Characteristics |
treatment: infected with Brn3b-lentiviruses
collecting day: day 2
|
| Treatment protocol |
MEFs were infected with Ascl1/Brn3b/Isl1 overexpressing lentiviruses, doxycycline was added on day 0 to induce Ascl1/Brn3b/Isl1 expression, cells were switched to neuronal medium (DMEM/F12+GlutaMAX+N2+B27+Forskolin+FGF2) on day 2 of reprogramming; medium was half-changed every other day.
|
| Growth protocol |
MEFs were cultured in DMEM+10% FBS, at 37℃ in incubator.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol following manufacturer's protocol
NEBNext UltraTM RNA library Prep Kit for Illumina following manufacturer's protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
d2 and d7-gene counts
|
| Data processing |
Basecalls performed using CASAVA
clean reads were aligned to the mouse reference genome (mm10) using Hisat2
featureCounts was used to count the reads numbers mapped to each genes
Genome_build: mm10
Supplementary_files_format_and_content: read counts for each genes were given in the processed data files
|
| |
|
| Submission date |
Nov 19, 2019 |
| Last update date |
Sep 30, 2020 |
| Contact name |
SHUYI CHEN |
| E-mail(s) |
chenshy23@mail.sysu.edu.cn
|
| Organization name |
Zhongshan Ophthalmic Center, SYSU
|
| Department |
State Key Laboratory of Ophthalmology
|
| Street address |
54 S. Xianlie Rd
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510060 |
| Country |
China |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE140689 |
Direct induction of retinal ganglion cell-like neurons |
|
| Relations |
| BioSample |
SAMN13331486 |
| SRA |
SRX7186708 |
