|
Status |
Public on Nov 17, 2009 |
Title |
Cerebellum left animal 5 |
Sample type |
other |
|
|
Channel 1 |
Source name |
Cerebellum left hand side
|
Organism |
Rattus norvegicus |
Characteristics |
strain: Sprague-Dawley gender: male developmental stage: Adult tissue: cerebellum hemisphere: Left molecule: microRNA
|
Growth protocol |
Animals were kept under normal day/night and dairy conditions with unlimited access to food and water.
|
Extracted molecule |
other |
Extraction protocol |
RNeasy Lipid Tissue Mini Kit (Qiagen) following manufacturer's instructions
|
Label |
Alexa Fluor® 5 fluorophor
|
Label protocol |
600 ng microRNA sample were poly-A tailed and tagged with the sequence tag for the Alexa Fluor® 5 fluorophor using the NCode™ miRNA Labeling System (Invitrogen). Universal reference were labeled with Alexa Fluor® 3.
|
|
|
Channel 2 |
Source name |
universal brain reference
|
Organism |
Rattus norvegicus |
Characteristics |
sample type: Invitrogen universal brain reference small RNA (>200 nt in length)
|
Extracted molecule |
other |
Extraction protocol |
RNeasy Lipid Tissue Mini Kit (Qiagen) following manufacturer's instructions
|
Label |
Alexa Fluor® 5 fluorophor
|
Label protocol |
600 ng microRNA sample were poly-A tailed and tagged with the sequence tag for the Alexa Fluor® 5 fluorophor using the NCode™ miRNA Labeling System (Invitrogen). Universal reference were labeled with Alexa Fluor® 3.
|
|
|
|
Hybridization protocol |
Samples were hybridized against the reference overnight to NCode™ Multi-Species miRNA Microarrays (Invitrogen, Carlsbad, CA, USA) using the NCode™ Multi-Species miRNA Microarrays Kit (Invitrogen, Carlsbad, CA, USA) following the instructions given by the manufacturer. Arrays were then hybridized on a MAUI hybridization station (BioMicro Systems, Inc, Salt Lake City, UT, USA) with Alexa Fluor® 3 and Alexa Fluor® 5 capture reagents and washed.
|
Scan protocol |
Scanned using an Agilent DNA microarray scanner. Images were processed using GenePix Pro software (Molecular Devices, Sunnyvale, CA, USA).
|
Description |
Biological replicate 4 of 4.
|
Data processing |
Background adjusted using “normexp” with an offset of 50. Print-tip-loess normalization was used to adjust data within each array and subsequent Gquantile normalization to allow comparisons between arrays. Thelimma package from BioConductor was used in R.
|
|
|
Submission date |
Jun 19, 2009 |
Last update date |
Nov 17, 2009 |
Contact name |
Thomas Werge |
Phone |
+45 4633 4968
|
Organization name |
Psychiatric Center, Sct. Hans Hospital
|
Department |
Institute of biological Psychiatry
|
Street address |
Boserupvej 2
|
City |
Roskilde |
ZIP/Postal code |
Dk-4000 |
Country |
Denmark |
|
|
Platform ID |
GPL8745 |
Series (1) |
GSE16725 |
Regional brain expression maps for microRNAs in rats |
|