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Sample GSM4221283 Query DataSets for GSM4221283
Status Public on Nov 17, 2020
Title WT 1
Sample type SRA
 
Source name Freshly isolated Cardiac Myocytes
Organism Mus musculus
Characteristics cell type: Freshly isolated Cardiac Myocytes
genotype: Control
Extracted molecule total RNA
Extraction protocol RNA-Sequencing (RNA-Seq): Total RNA was isolated from cardiac myocytes of 2-week old WT and Myh6-Cre:LmnaF/F mice. RNA-seq was performed on the on ribosome-depleted RNA.
Samples with a RNA Integrity Number (RIN) read out of more than 8 were used to prepare sequencing library using the Illumina TruSeq stranded total RNA library preparation kit. The samples were sequenced on the Illumina HiSeq 4000 instrument using the paired-end sequencing reagents to generate 100 base pair runs.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 4000
 
Description NTG_Cm_1
Data processing Fastq files where trimmed using TrimGalore!.
Trimmed files were aligned using HISAT2.
Genes were quantified using featureCount.
Genome_build: GRCM38
Supplementary_files_format_and_content: The processed data file is in a comma seperated format and contains the final featureCount generated count matrix.
 
Submission date Dec 16, 2019
Last update date Nov 17, 2020
Contact name Ali J Marian
E-mail(s) Ali.J.Marian@uth.tmc.edu
Phone 713-500-2350
Organization name UTHSC, Houston
Department Centre for Cardiovascular Genetics
Lab Marian Lab
Street address 6770 Bertner Ave DAC C900a
City Houaton
State/province Texas
ZIP/Postal code 77030
Country USA
 
Platform ID GPL21103
Series (2)
GSE142125 Inhibition of BRD4 Partially Rescues Dilated Cardiomyopathy and Cardiac Arrhythmias in Myocyte-Specific Lamin A/C Null Mice [RNA-Seq]
GSE142129 Inhibition of BRD4 Partially Rescues Dilated Cardiomyopathy and Cardiac Arrhythmias in Myocyte-Specific Lamin A/C Null Mice
Relations
BioSample SAMN13575975
SRA SRX7388453

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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