|
Status |
Public on Nov 17, 2020 |
Title |
WT 3 |
Sample type |
SRA |
|
|
Source name |
Freshly isolated Cardiac Myocytes
|
Organism |
Mus musculus |
Characteristics |
cell type: Freshly isolated Cardiac Myocytes genotype: Control
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA-Sequencing (RNA-Seq): Total RNA was isolated from cardiac myocytes of 2-week old WT and Myh6-Cre:LmnaF/F mice. RNA-seq was performed on the on ribosome-depleted RNA. Samples with a RNA Integrity Number (RIN) read out of more than 8 were used to prepare sequencing library using the Illumina TruSeq stranded total RNA library preparation kit. The samples were sequenced on the Illumina HiSeq 4000 instrument using the paired-end sequencing reagents to generate 100 base pair runs.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 4000 |
|
|
Description |
NTG_Cm_4
|
Data processing |
Fastq files where trimmed using TrimGalore!. Trimmed files were aligned using HISAT2. Genes were quantified using featureCount. Genome_build: GRCM38 Supplementary_files_format_and_content: The processed data file is in a comma seperated format and contains the final featureCount generated count matrix.
|
|
|
Submission date |
Dec 16, 2019 |
Last update date |
Nov 17, 2020 |
Contact name |
Ali J Marian |
E-mail(s) |
Ali.J.Marian@uth.tmc.edu
|
Phone |
713-500-2350
|
Organization name |
UTHSC, Houston
|
Department |
Centre for Cardiovascular Genetics
|
Lab |
Marian Lab
|
Street address |
6770 Bertner Ave DAC C900a
|
City |
Houaton |
State/province |
Texas |
ZIP/Postal code |
77030 |
Country |
USA |
|
|
Platform ID |
GPL21103 |
Series (2) |
GSE142125 |
Inhibition of BRD4 Partially Rescues Dilated Cardiomyopathy and Cardiac Arrhythmias in Myocyte-Specific Lamin A/C Null Mice [RNA-Seq] |
GSE142129 |
Inhibition of BRD4 Partially Rescues Dilated Cardiomyopathy and Cardiac Arrhythmias in Myocyte-Specific Lamin A/C Null Mice |
|
Relations |
BioSample |
SAMN13575973 |
SRA |
SRX7388455 |