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Sample GSM422393 Query DataSets for GSM422393
Status Public on Jan 01, 2010
Title Adult testis, wild-type, replicate 3
Sample type RNA
 
Source name Mouse testis, adult, wildtype
Organism Mus musculus
Characteristics tissue: testis
genotype: wild type
developmental stage: adult
Treatment protocol Ovaries/testes were removed from euthanized animals after perfusion with PBS and snap-frozen in liquid nitrogen (no perfusion for P3). Adult expression profiles represent single organ samples (single animals), whereas P3 profiles represent pools of >10 females per chip.
Growth protocol Mice were housed in specific pathogen-free controlled conditions. Food and water were available ad libitum. All experiments were carried out in accordance with the principles and guidelines of the LAR/European Molecular Biology Laboratory (EMBL).
Extracted molecule total RNA
Extraction protocol RNeasy (Qiagen) RNA isolation from animal tissues according to standard protocol, including on-column DNase digestion.
Label biotin
Label protocol Biotinylated cRNAs were prepared according to the standard GeneChip WT Sense Target Labeling Assay Manual protocol (Affymetrix) from 300ng total RNA. For the P3 samples, 1µg RNA was labeled according to the CodeLink Bioarray Processing Protocol (Amersham).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 17hr at 45C on Affymetrix Mouse Genome 430 2.0 Arrays (P3) and Affymetrix Mouse Gene 1.0 ST Arrays (Adult). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix Scanner 3000 7G.
Description Expression profiles of adult testes
Data processing Data were analyzed with RMA using Affymetrix default analysis settings (Expression Console). Up- and downregulated genes were identified using the GeneSpring GX statistical software package (Agilent).
 
Submission date Jun 26, 2009
Last update date Jun 26, 2009
Contact name Henriette Uhlenhaut
E-mail(s) henriette.uhlenhaut@embl.de, nuhlenhaut@salk.edu
Phone +496221387432
URL http://www.embl.org
Organization name EMBL
Department Developmental Biology Unit
Lab Treier
Street address Meyerhofstrasse 1
City Heidelberg
ZIP/Postal code 69117
Country Germany
 
Platform ID GPL6246
Series (1)
GSE16853 Expression data from Foxl2 wild-type and mutant ovaries and testes

Data table header descriptions
ID_REF
VALUE RMA signal

Data table
ID_REF VALUE
10344614 5.453424
10344616 1.685721
10344620 2.796141
10344622 6.471695
10344624 9.188391
10344633 8.574396
10344637 7.663575
10344653 2.880828
10344658 9.033968
10344674 2.597485
10344679 4.193165
10344707 4.477814
10344713 8.05031
10344715 2.655981
10344717 3.386147
10344719 4.542604
10344721 1.705441
10344723 7.07659
10344725 5.82134
10344741 8.968285

Total number of rows: 35557

Table truncated, full table size 621 Kbytes.




Supplementary file Size Download File type/resource
GSM422393.CEL.gz 6.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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