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Sample GSM422395 Query DataSets for GSM422395
Status Public on Jan 01, 2010
Title Adult ovary, wild-type, replicate 2
Sample type RNA
Source name Mouse ovary, adult, wildtype
Organism Mus musculus
Characteristics tissue: ovary
genotype: wild type
developmental stage: adult
Treatment protocol Ovaries/testes were removed from euthanized animals after perfusion with PBS and snap-frozen in liquid nitrogen (no perfusion for P3). Adult expression profiles represent single organ samples (single animals), whereas P3 profiles represent pools of >10 females per chip.
Growth protocol Mice were housed in specific pathogen-free controlled conditions. Food and water were available ad libitum. All experiments were carried out in accordance with the principles and guidelines of the LAR/European Molecular Biology Laboratory (EMBL).
Extracted molecule total RNA
Extraction protocol RNeasy (Qiagen) RNA isolation from animal tissues according to standard protocol, including on-column DNase digestion.
Label biotin
Label protocol Biotinylated cRNAs were prepared according to the standard GeneChip WT Sense Target Labeling Assay Manual protocol (Affymetrix) from 300ng total RNA. For the P3 samples, 1µg RNA was labeled according to the CodeLink Bioarray Processing Protocol (Amersham).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 17hr at 45C on Affymetrix Mouse Genome 430 2.0 Arrays (P3) and Affymetrix Mouse Gene 1.0 ST Arrays (Adult). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix Scanner 3000 7G.
Description Expression profiles of adult ovaries
Data processing Data were analyzed with RMA using Affymetrix default analysis settings (Expression Console). Up- and downregulated genes were identified using the GeneSpring GX statistical software package (Agilent).
Submission date Jun 26, 2009
Last update date Jun 26, 2009
Contact name Henriette Uhlenhaut
Phone +496221387432
Organization name EMBL
Department Developmental Biology Unit
Lab Treier
Street address Meyerhofstrasse 1
City Heidelberg
ZIP/Postal code 69117
Country Germany
Platform ID GPL6246
Series (1)
GSE16853 Expression data from Foxl2 wild-type and mutant ovaries and testes

Data table header descriptions
VALUE RMA signal

Data table
10344614 4.942142
10344616 1.895239
10344620 2.767049
10344622 5.526515
10344624 7.836484
10344633 9.047528
10344637 7.937983
10344653 2.951859
10344658 6.734761
10344674 2.528474
10344679 4.102691
10344707 6.412553
10344713 9.122211
10344715 2.699018
10344717 4.305869
10344719 4.886057
10344721 2.034599
10344723 9.673499
10344725 6.923607
10344741 9.421892

Total number of rows: 35557

Table truncated, full table size 621 Kbytes.

Supplementary file Size Download File type/resource
GSM422395.cel.gz 6.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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