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Sample GSM422400 Query DataSets for GSM422400
Status Public on Jan 01, 2010
Title Ovary at P3, wild-type, replicate 2
Sample type RNA
 
Source name Mouse ovary, P3, wildtype
Organism Mus musculus
Characteristics tissue: ovary
genotype: wild type
developmental stage: newborn
Treatment protocol Ovaries/testes were removed from euthanized animals after perfusion with PBS and snap-frozen in liquid nitrogen (no perfusion for P3). Adult expression profiles represent single organ samples (single animals), whereas P3 profiles represent pools of >10 females per chip.
Growth protocol Mice were housed in specific pathogen-free controlled conditions. Food and water were available ad libitum. All experiments were carried out in accordance with the principles and guidelines of the LAR/European Molecular Biology Laboratory (EMBL).
Extracted molecule total RNA
Extraction protocol RNeasy (Qiagen) RNA isolation from animal tissues according to standard protocol, including on-column DNase digestion.
Label biotin
Label protocol Biotinylated cRNAs were prepared according to the standard GeneChip WT Sense Target Labeling Assay Manual protocol (Affymetrix) from 300ng total RNA. For the P3 samples, 1µg RNA was labeled according to the CodeLink Bioarray Processing Protocol (Amersham).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 17hr at 45C on Affymetrix Mouse Genome 430 2.0 Arrays (P3) and Affymetrix Mouse Gene 1.0 ST Arrays (Adult). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix Scanner 3000 7G.
Description Expression profiles of neonatal (P3) ovaries
Data processing MAS 5
 
Submission date Jun 26, 2009
Last update date Jun 26, 2009
Contact name Henriette Uhlenhaut
E-mail(s) henriette.uhlenhaut@embl.de, nuhlenhaut@salk.edu
Phone +496221387432
URL http://www.embl.org
Organization name EMBL
Department Developmental Biology Unit
Lab Treier
Street address Meyerhofstrasse 1
City Heidelberg
ZIP/Postal code 69117
Country Germany
 
Platform ID GPL1261
Series (1)
GSE16853 Expression data from Foxl2 wild-type and mutant ovaries and testes

Data table header descriptions
ID_REF
VALUE MAS 5 signal
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 817.7 P 0.000052
AFFX-BioB-M_at 1373.2 P 0.000044
AFFX-BioB-3_at 816.7 P 0.000044
AFFX-BioC-5_at 2959.5 P 0.000044
AFFX-BioC-3_at 3559.7 P 0.000044
AFFX-BioDn-5_at 5677.6 P 0.000044
AFFX-BioDn-3_at 12339.4 P 0.000044
AFFX-CreX-5_at 31463 P 0.000052
AFFX-CreX-3_at 34646.2 P 0.000044
AFFX-DapX-5_at 35.2 P 0.046482
AFFX-DapX-M_at 21 A 0.41138
AFFX-DapX-3_at 13.6 A 0.529762
AFFX-LysX-5_at 4 A 0.645559
AFFX-LysX-M_at 6.2 A 0.699394
AFFX-LysX-3_at 3.7 A 0.470238
AFFX-PheX-5_at 2.2 A 0.984817
AFFX-PheX-M_at 1.6 A 0.978098
AFFX-PheX-3_at 8.5 A 0.824672
AFFX-ThrX-5_at 20.3 A 0.39692
AFFX-ThrX-M_at 2.5 A 0.749204

Total number of rows: 45101

Table truncated, full table size 1216 Kbytes.




Supplementary file Size Download File type/resource
GSM422400.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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