Total RNA was isolated from ten 10-μm sections of FFPE samples using the Roche high pure RNA FFPE isolation kit.
Label
Nanostring
Label protocol
standard Nanostring protocol
Hybridization protocol
none provided by submitter
Scan protocol
50-100 ng of RNA was analysed for the expression of 770 genes related to the innate immune system with the nCounter® Human Myeloid Innate Immunity Panel v2 utilizing the NanoString platform, using the CodeSet-Plus/Panel-Plus nCounter XT Gene Expression Assay.
Data processing
Raw data were quality checked in the nSolver software followed by normalization. Normalization factors were calculated based on 40 reference genes included in the myeloid innate immunity panel.
