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Sample GSM4261267 Query DataSets for GSM4261267
Status Public on Jul 30, 2020
Title RNAseq_Ad_lib_WT_ZT19_rep2
Sample type SRA
Source name Liver
Organism Mus musculus
Characteristics strain: C57BL/6J
gender: male
diet: normal chow
genotype/variation: WT
tissue: Liver
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from liver tissue or isolated endothelial cells and kupffer cells using Trizol reagent followed by RNeasy Mini Kit (#74104).
Libraries were prepared according to Illumina's instructions accompanying the TruSeq RNA Sample Preparation. The purified cDNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
Description Ad_lib_feeding
Data processing RNA-seq reads were aligned to the University of California, Santa Cruz (UCSC) mm9 genome browser using STAR. The tag directories were established and normalized read counts were measured with RefSeq genes using Homer.
Genome_build: mm9
Submission date Jan 13, 2020
Last update date Jul 30, 2020
Contact name Dongyin Guan
Organization name Baylor College of Medicine
Lab Dongyin Guan
Street address 1 Baylor Plaza, ABBR
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
Platform ID GPL17021
Series (2)
GSE143524 The Hepatocyte Clock and Feeding Interdependently Control Chrono-Homeostasis of Multiple Liver Cell Types (RNA-seq)
GSE143528 The hepatocyte clock and feeding control chronophysiology of multiple liver cell types
BioSample SAMN13836318
SRA SRX7546493

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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