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Sample GSM4277332 Query DataSets for GSM4277332
Status Public on Dec 07, 2020
Title 13etoh3
Sample type SRA
 
Source name murine ESC
Organism Mus musculus
Characteristics genotype/variation: -5 floxed, exogenous mNanogV5
treatment: Vehicle
Treatment protocol 6 days of ethanol (vehicle) or tamoxifen (4OHT)
Growth protocol 200K cells were plated in 6 well plates
Extracted molecule polyA RNA
Extraction protocol RNA was extracted using Trizol, processed through the Qiagen RNAeasy Mini Plus Kit
RNA-seq libraries were made using the NEBNext Ultra RNA Library Prep Kit for Illumina
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Description 5del_DESeq.gct
Data processing Reads were aligned using STAR to mm9
Aligned reads were analyzed using DESeq
Genome_build: mm9
 
Submission date Jan 20, 2020
Last update date Dec 07, 2020
Contact name Sridhar Rao
E-mail(s) sridhar.rao@bcw.edu
Phone 4149373841
Organization name BloodCenter of Wisconsin
Department Blood Research Institute
Street address 8727 Watertown Plank Road
City Milwaukee
State/province WI
ZIP/Postal code 53226
Country USA
 
Platform ID GPL19057
Series (2)
GSE143959 Defining a critical Nanog enhancer using chromatin-focused approaches identifies RNAPII recruitment as required for expression [-5delRNASeq]
GSE143993 Genome editing demonstrates that the -5 kb Nanog enhancer regulates Nanog expression by modulating RNAPII initiation and/or recruitment
Relations
BioSample SAMN13892711
SRA SRX7584352

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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