|
Status |
Public on Jan 10, 2021 |
Title |
wt3 |
Sample type |
SRA |
|
|
Source name |
murine ESC
|
Organism |
Mus musculus |
Characteristics |
genotype/variation: wildtype
|
Treatment protocol |
6 days of ethanol (vehicle) or tamoxifen (4OHT)
|
Growth protocol |
200K cells were plated in 6 well plates
|
Extracted molecule |
polyA RNA |
Extraction protocol |
RNA was extracted using Trizol, processed through the Qiagen RNAeasy Mini Plus Kit RNA-seq libraries were made using the NEBNext Ultra RNA Library Prep Kit for Illumina
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Description |
DEXSeq_exon_counts.csv
|
Data processing |
Reads were aligned using STAR to mm9 Aligned reads were analyzed using DEXeq Genome_build: mm9
|
|
|
Submission date |
Jan 20, 2020 |
Last update date |
Jan 10, 2021 |
Contact name |
Sridhar Rao |
E-mail(s) |
sridhar.rao@bcw.edu
|
Phone |
4149373841
|
Organization name |
BloodCenter of Wisconsin
|
Department |
Blood Research Institute
|
Street address |
8727 Watertown Plank Road
|
City |
Milwaukee |
State/province |
WI |
ZIP/Postal code |
53226 |
Country |
USA |
|
|
Platform ID |
GPL19057 |
Series (1) |
GSE143960 |
Two super-enhancers regulate Nanog expression through distinct mechanisms [45del] |
|
Relations |
BioSample |
SAMN13892716 |
SRA |
SRX7584358 |