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Sample GSM4277800 Query DataSets for GSM4277800
Status Public on Dec 07, 2020
Title wt_2
Sample type SRA
Source name murine ESC
Organism Mus musculus
Characteristics genotype/variation: wildtype
antibody: RNA Polymerase II Ser5P CUT&Tag
Growth protocol cells grown in 6 well dishes
Extracted molecule genomic DNA
Extraction protocol Cells were processed according to Kaya-Okur et al., 2019, using Antibody to RNA Polymerase II - Ser5P (Cell Signaling Technologies #13523)
Libraries were made according to Kaya-Okur et al., 2019
Library strategy OTHER
Library source genomic
Library selection other
Instrument model Illumina NextSeq 500
Description wt_Ser5P_cutntag.bedgraph
Data processing Library strategy: CUT&Tag
Reads were aligned using Bowtie2 (v2.3.4.3)
replicates were concatenated
Genome_build: mm9
Submission date Jan 21, 2020
Last update date Dec 07, 2020
Contact name Sridhar Rao
Phone 4149373841
Organization name BloodCenter of Wisconsin
Department Blood Research Institute
Street address 8727 Watertown Plank Road
City Milwaukee
State/province WI
ZIP/Postal code 53226
Country USA
Platform ID GPL19057
Series (2)
GSE143991 Defining a critical Nanog enhancer using chromatin-focused approaches identifies RNAPII recruitment as required for expression [CUT&Tag]
GSE143993 Genome editing demonstrates that the -5 kb Nanog enhancer regulates Nanog expression by modulating RNAPII initiation and/or recruitment
BioSample SAMN13895717
SRA SRX7586111

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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