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Sample GSM4277800 Query DataSets for GSM4277800
Status Public on Dec 07, 2020
Title wt_2
Sample type SRA
 
Source name murine ESC
Organism Mus musculus
Characteristics genotype/variation: wildtype
antibody: RNA Polymerase II Ser5P CUT&Tag
Growth protocol cells grown in 6 well dishes
Extracted molecule genomic DNA
Extraction protocol Cells were processed according to Kaya-Okur et al., 2019, using Antibody to RNA Polymerase II - Ser5P (Cell Signaling Technologies #13523)
Libraries were made according to Kaya-Okur et al., 2019
 
Library strategy OTHER
Library source genomic
Library selection other
Instrument model Illumina NextSeq 500
 
Description wt_Ser5P_cutntag.bedgraph
Data processing Library strategy: CUT&Tag
Reads were aligned using Bowtie2 (v2.3.4.3)
replicates were concatenated
Genome_build: mm9
 
Submission date Jan 21, 2020
Last update date Dec 07, 2020
Contact name Sridhar Rao
E-mail(s) sridhar.rao@bcw.edu
Phone 4149373841
Organization name BloodCenter of Wisconsin
Department Blood Research Institute
Street address 8727 Watertown Plank Road
City Milwaukee
State/province WI
ZIP/Postal code 53226
Country USA
 
Platform ID GPL19057
Series (2)
GSE143991 Defining a critical Nanog enhancer using chromatin-focused approaches identifies RNAPII recruitment as required for expression [CUT&Tag]
GSE143993 Genome editing demonstrates that the -5 kb Nanog enhancer regulates Nanog expression by modulating RNAPII initiation and/or recruitment
Relations
BioSample SAMN13895717
SRA SRX7586111

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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