|
Status |
Public on Dec 07, 2020 |
Title |
0copy_1 |
Sample type |
SRA |
|
|
Source name |
murine ESC
|
Organism |
Mus musculus |
Characteristics |
genotype/variation: -5 deleted with mNanogV5 antibody: RNA Polymerase II Ser5P CUT&Tag
|
Growth protocol |
cells grown in 6 well dishes
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Cells were processed according to Kaya-Okur et al., 2019, using Antibody to RNA Polymerase II - Ser5P (Cell Signaling Technologies #13523) Libraries were made according to Kaya-Okur et al., 2019
|
|
|
Library strategy |
OTHER |
Library source |
genomic |
Library selection |
other |
Instrument model |
Illumina NextSeq 500 |
|
|
Description |
0copy_Ser5P_cutntag.bedgraph
|
Data processing |
Library strategy: CUT&Tag Reads were aligned using Bowtie2 (v2.3.4.3) replicates were concatenated Genome_build: mm9
|
|
|
Submission date |
Jan 21, 2020 |
Last update date |
Dec 07, 2020 |
Contact name |
Sridhar Rao |
E-mail(s) |
sridhar.rao@bcw.edu
|
Phone |
4149373841
|
Organization name |
BloodCenter of Wisconsin
|
Department |
Blood Research Institute
|
Street address |
8727 Watertown Plank Road
|
City |
Milwaukee |
State/province |
WI |
ZIP/Postal code |
53226 |
Country |
USA |
|
|
Platform ID |
GPL19057 |
Series (2) |
GSE143991 |
Defining a critical Nanog enhancer using chromatin-focused approaches identifies RNAPII recruitment as required for expression [CUT&Tag] |
GSE143993 |
Genome editing demonstrates that the -5 kb Nanog enhancer regulates Nanog expression by modulating RNAPII initiation and/or recruitment |
|
Relations |
BioSample |
SAMN13895711 |
SRA |
SRX7586117 |