|
| Status |
Public on Jul 31, 2020 |
| Title |
[CuR+Pip] + AB Rep 2: Combined curcumin and piperine treatment followed by addition of Aβ, biology rep2 |
| Sample type |
RNA |
| |
|
| Source name |
SH-SY5Y Neuroblastoma Cell line
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: bone marrow
gender: female
cell line: SH-SY5Y
cell type: Neuroblastoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
100ng of total RNA was reverse transcribed to produce cDNA/mRNA hybrid molecule, which was subsequently used as a template to create double stranded cDNA. This double-stranded cDNA was then amplified via in vitro transcription (IVT) to produce cRNA.
|
| |
|
| Hybridization protocol |
In vitro transcription (IVT) generated cRNA was then purified and subjected to 2nd-cycle single-stranded sense cDNA synthesis which was later fragmented, labeled, and hybridized to Human Clariom S Array for 16 hours at 45°C with rotation at 60 rpm.
|
| Scan protocol |
Arrays were then washed and stained using the FS450_0007 fluidics protocol and scanned using an Applied Biosystems™ GeneChip™ Scanner 3000 7G.
|
| Description |
Gene expression data from cells treated with combined compound of curcumin and piperine followed by addition of amyloid beta
|
| Data processing |
The scanned images were inspected for hybridization efficiency and CEL files generated from GeneChip Command Console Software were imported into Transcriptome Analysis Console v4.0 software for array QC. RMA normalization was performed on the samples to generate the quality control (QC) metrics that we routinely use to determine data quality. These include, All Probeset mean, Background mean (Bgrd_Mean), positive and negative probes (POS vs NEG AUC), bacterial spike controls and Poly-A controls.
|
| |
|
| Submission date |
Jan 21, 2020 |
| Last update date |
Jul 31, 2020 |
| Contact name |
Aimi Syamima Abdul Manap |
| E-mail(s) |
amymia276@gmail.com
|
| Organization name |
Taylor's University
|
| Street address |
No. 1, Jalan Taylor's
|
| City |
Subang Jaya |
| State/province |
Selangor |
| ZIP/Postal code |
47500 |
| Country |
Malaysia |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE143998 |
Whole transcript analysis of amyloid beta 42 (Aβ42)-induced SH-SY5Y cells in control and treated groups |
|
