|
| Status |
Public on Mar 31, 2010 |
| Title |
LLC-PSF1P-EGFP_low, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
LLC-PSF1P-EGFP xenograft tumor
|
| Organism |
Mus musculus |
| Characteristics |
cell line: lewis lung carcinoma (LLC)
psf1 expression: low
biological replicate: 1
tumor stage: 4 weeks after innocuation
innocuation site: s.c.
|
| Growth protocol |
LLC-PSF1P-EGFP or colon26-PSF1P-EGFP were innocuated into s.c. of mice and disected after 4(LLC-PSF1P-EGFP) or 2(colon26-PSF1P-EGFP) weeks after innocuation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation,10 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the AFFYMETRIX GeneChip Scanner 7G
|
| Description |
Gene expression data from sorted PSF1 low expression cells of LLC-PSF1P-EGFP
|
| Data processing |
The data were analyzed with RMA using R/Bioconductor and global scaling as normalization method.
|
| |
|
| Submission date |
Jul 15, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Nobuyuki Takakura |
| E-mail(s) |
ntakaku@biken.osaka-u.ac.jp
|
| Phone |
81-6-6879-8312
|
| Fax |
81-6-6879-8314
|
| Organization name |
The Research Institute for Microbial Disease, Osaka university
|
| Department |
Signal Transduction
|
| Street address |
Yamada-oka 3-1
|
| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE17112 |
Visualization of cancer initiating cell in the vascular niche utilized with transcriptional activity of PSF1 |
|
| Relations |
| Reanalyzed by |
GSE119085 |
