|
Status |
Public on Dec 07, 2020 |
Title |
Healthy_skin_sample_#2 (miRNA) |
Sample type |
RNA |
|
|
Source name |
skin
|
Organism |
Homo sapiens |
Characteristics |
disease state: control tissue: skin
|
Treatment protocol |
3 total skin samples from patients with psoriasis and 3 samples from healthy controls were analyzed (miRNA)
|
Growth protocol |
NA
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
200ng of total RNA were labeled using the FlashTag Biotin HSR RNA Labeling Kit Kit (Catalog Number 901910, Affymetrix, Santa Clara, CA)
|
|
|
Hybridization protocol |
Affymetrix Human miRNA 4.0 arrays were hybridized, washed, stained and scanned according to the protocol described in FlashTag Biotin HSR RNA Labeling Kit (P/N 703095 Rev.2) User Manual
|
Scan protocol |
Scanning was done on an Affymetrix GeneChip Scanner 3000 7G, software AGCC 3.1.1
|
Data processing |
The data were analyzed withTranscriptome Analysis Console (TAC) 4.0.1 using default analysis settings. Row data were log2-transformed and normalized by Robust multichip average (RMA) algorithm normalization.
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|
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Submission date |
Feb 10, 2020 |
Last update date |
Dec 08, 2020 |
Contact name |
Markus Jeitler |
E-mail(s) |
markus.jeitler@meduniwien.ac.at
|
Organization name |
Medical University Vienna
|
Street address |
Lazarettgasse 14
|
City |
Vienna |
ZIP/Postal code |
A-1090 |
Country |
Austria |
|
|
Platform ID |
GPL19117 |
Series (2) |
GSE145054 |
Combined miRNA-mRNA transcriptional profiling in the human skin [miRNA_PSOR] |
GSE145305 |
Combined miRNA-mRNA transcriptional profiling in the human skin |
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