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Sample GSM4305476 Query DataSets for GSM4305476
Status Public on Dec 07, 2020
Title Healthy_skin_sample_#4 (miRNA)
Sample type RNA
Source name skin
Organism Homo sapiens
Characteristics disease state: control
tissue: skin
Treatment protocol 3 total skin samples from patients with psoriasis and 3 samples from healthy controls were analyzed (miRNA)
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol 200ng of total RNA were labeled using the FlashTag Biotin HSR RNA Labeling Kit Kit (Catalog Number 901910, Affymetrix, Santa Clara, CA)
Hybridization protocol Affymetrix Human miRNA 4.0 arrays were hybridized, washed, stained and scanned according to the protocol described in FlashTag Biotin HSR RNA Labeling Kit (P/N 703095 Rev.2) User Manual
Scan protocol Scanning was done on an Affymetrix GeneChip Scanner 3000 7G, software AGCC 3.1.1
Data processing The data were analyzed withTranscriptome Analysis Console (TAC) 4.0.1 using default analysis settings. Row data were log2-transformed and normalized by Robust multichip average (RMA) algorithm normalization.
Submission date Feb 10, 2020
Last update date Dec 08, 2020
Contact name Markus Jeitler
Organization name Medical University Vienna
Street address Lazarettgasse 14
City Vienna
ZIP/Postal code A-1090
Country Austria
Platform ID GPL19117
Series (2)
GSE145054 Combined miRNA-mRNA transcriptional profiling in the human skin [miRNA_PSOR]
GSE145305 Combined miRNA-mRNA transcriptional profiling in the human skin

Data table header descriptions
VALUE normalized RMA expression values

Data table
MIMAT0021827_st 8.64
MIMAT0022528_st 8.06
MIMAT0004123_st 8.88
MIMAT0024174_st 8.88
MIMAT0008362_st 3.63
MIMAT0000223_st 8.56
MIMAT0000459_st 8.56
MIMAT0000868_st 8.56
MIMAT0001856_st 8.56
MIMAT0002969_st 8.56
MIMAT0002970_st 8.56
MIMAT0003795_st 8.56
MIMAT0007740_st 8.56
MIMAT0006226_st 8.56
MIMAT0008058_st 8.56
MIMAT0013026_st 8.56
MIMAT0013895_st 8.56
MIMAT0015788_st 8.56
MIMAT0026251_st 8.56
MIMAT0003528_st 10.73

Total number of rows: 36222

Table truncated, full table size 733 Kbytes.

Supplementary file Size Download File type/resource
GSM4305476_MJ_11082018_MIL_10_miRNA-4_0_.CEL.gz 809.0 Kb (ftp)(http) CEL
Processed data included within Sample table

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