|
| Status |
Public on Dec 07, 2020 |
| Title |
differentiated_keratinocyte_#3 (miRNA) |
| Sample type |
RNA |
| |
|
| Source name |
keratinocytes
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: healthy
cell type: differentiated keratinocyte
|
| Treatment protocol |
from each conditation (pKC, dKC, SE) one samples from an individual healy participan was analysed
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
200ng of total RNA were labeled using the FlashTag Biotin HSR RNA Labeling Kit Kit (Catalog Number 901910, Affymetrix, Santa Clara, CA)
|
| |
|
| Hybridization protocol |
Affymetrix Human miRNA 4.0 arrays were hybridized, washed, stained and scanned according to the protocol described in FlashTag Biotin HSR RNA Labeling Kit (P/N 703095 Rev.2) User Manual
|
| Scan protocol |
Scanning was done on an Affymetrix GeneChip Scanner 3000 7G, software AGCC 3.1.1
|
| Description |
dKC#3
|
| Data processing |
The data were analyzed withTranscriptome Analysis Console (TAC) 4.0.1 using default analysis settings. Row data were log2-transformed and normalized by Robust multichip average (RMA) algorithm normalization.
|
| |
|
| Submission date |
Feb 10, 2020 |
| Last update date |
Dec 08, 2020 |
| Contact name |
Markus Jeitler |
| E-mail(s) |
markus.jeitler@meduniwien.ac.at
|
| Organization name |
Medical University Vienna
|
| Street address |
Lazarettgasse 14
|
| City |
Vienna |
| ZIP/Postal code |
A-1090 |
| Country |
Austria |
| |
|
| Platform ID |
GPL19117 |
| Series (2) |
| GSE145056 |
Combined miRNA-mRNA transcriptional profiling in the human skin [miRNA_Kera] |
| GSE145059 |
Combined miRNA-mRNA transcriptional profiling in the human skin [Kera] |
|
