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Sample GSM4305521 Query DataSets for GSM4305521
Status Public on Dec 07, 2020
Title skin_equivalent_#3 (miRNA)
Sample type RNA
 
Source name keratinocytes
Organism Homo sapiens
Characteristics disease state: healthy
cell type: skin equivalent keratinocyte
Treatment protocol from each conditation (pKC, dKC, SE) one samples from an individual healy participan was analysed
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol 200ng of total RNA were labeled using the FlashTag Biotin HSR RNA Labeling Kit Kit (Catalog Number 901910, Affymetrix, Santa Clara, CA)
 
Hybridization protocol Affymetrix Human miRNA 4.0 arrays were hybridized, washed, stained and scanned according to the protocol described in FlashTag Biotin HSR RNA Labeling Kit (P/N 703095 Rev.2) User Manual
Scan protocol Scanning was done on an Affymetrix GeneChip Scanner 3000 7G, software AGCC 3.1.1
Description SE#3
Data processing The data were analyzed withTranscriptome Analysis Console (TAC) 4.0.1 using default analysis settings. Row data were log2-transformed and normalized by Robust multichip average (RMA) algorithm normalization.
 
Submission date Feb 10, 2020
Last update date Dec 08, 2020
Contact name Markus Jeitler
E-mail(s) markus.jeitler@meduniwien.ac.at
Organization name Medical University Vienna
Street address Lazarettgasse 14
City Vienna
ZIP/Postal code A-1090
Country Austria
 
Platform ID GPL19117
Series (2)
GSE145056 Combined miRNA-mRNA transcriptional profiling in the human skin [miRNA_Kera]
GSE145059 Combined miRNA-mRNA transcriptional profiling in the human skin [Kera]

Data table header descriptions
ID_REF
VALUE normalized RMA expression values

Data table
ID_REF VALUE
MIMAT0020884_st 6.65
MIMAT0012432_st 6.65
MIMAT0002123_st 10.1
MIMAT0002266_st 10.1
MIMAT0002267_st 10.1
MIMAT0002268_st 10.1
MIMAT0002269_st 10.1
MIMAT0002270_st 10.1
MIMAT0003688_st 10.1
MIMAT0016146_st 5.03
MIMAT0027347_st 4.62
MIMAT0016373_st 5.05
MIMAT0012321_st 10.18
MIMAT0004978_st 4.22
MIMAT0008342_st 4.22
MIMAT0009385_st 4.22
MIMAT0012845_st 4.22
MIMAT0013947_st 4.22
MIMAT0027981_st 5.88
MIMAT0028116_st 4.98

Total number of rows: 36222

Table truncated, full table size 734 Kbytes.




Supplementary file Size Download File type/resource
GSM4305521_MJ_10292014_MIL_9_miRNA-4_0_.CEL.gz 867.9 Kb (ftp)(http) CEL
Processed data included within Sample table

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