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Sample GSM4305546 Query DataSets for GSM4305546
Status Public on Dec 07, 2020
Title skin_equivalent_#3 (mRNA)
Sample type RNA
 
Source name keratinocytes
Organism Homo sapiens
Characteristics disease state: healthy
cell type: skin equivalent keratinocyte
Treatment protocol from each conditation (pKC, dKC, SE) one samples from an individual healy participan was analysed
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions and subsequent RNA cleanup and concentration was performed using the Rneasy MinElure Cleanup Kit (Qiagen, Venlo, Netherlands)
Label biotin
Label protocol 200ng of total RNA were amplified and labeled using the GeneChip® WT PLUS Reagent Kit (Catalog Number 902281, Affymetrix, Santa Clara, CA)
 
Hybridization protocol Affymetrix Human Gene 2.0 ST arrays were washed, stained and scanned according to the protocol described in GeneChip® WT Terminal Labeling and Hybridization User Manual (Fluidics Protocol FS450_0007)
Scan protocol Scanning was done on an Affymetrix GeneChip Scanner 3000 7G, software AGCC 3.1.1
Description SE#3
Data processing The data were analyzed withTranscriptome Analysis Console (TAC) 4.0.1 using default analysis settings. Row data were log2-transformed and normalized by Robust multichip average (RMA) algorithm normalization.
 
Submission date Feb 10, 2020
Last update date Dec 08, 2020
Contact name Markus Jeitler
E-mail(s) markus.jeitler@meduniwien.ac.at
Organization name Medical University Vienna
Street address Lazarettgasse 14
City Vienna
ZIP/Postal code A-1090
Country Austria
 
Platform ID GPL16686
Series (2)
GSE145058 Combined miRNA-mRNA transcriptional profiling in the human skin [mRNA_Kera]
GSE145059 Combined miRNA-mRNA transcriptional profiling in the human skin [Kera]

Data table header descriptions
ID_REF
VALUE normalized RMA expression values

Data table
ID_REF VALUE
16657436 5.28
16657440 4.73
16657445 2.68
16657447 1.88
16657450 5.71
16657469 5.29
16657473 2.22
16657476 5.82
16657480 3
16657485 2.15
16657489 4.5
16657492 3.95
16657502 3.41
16657506 4.09
16657509 3.25
16657514 4.65
16657527 3.95
16657529 3.77
16657534 5.43
16657554 5.77

Total number of rows: 48226

Table truncated, full table size 654 Kbytes.




Supplementary file Size Download File type/resource
GSM4305546_MJ_10142014_MIL_9_HuGene-2_0-st_.CEL.gz 7.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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