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Sample GSM4314529 Query DataSets for GSM4314529
Status Public on Dec 07, 2020
Title Psoriasis_skin_sample_#1 (mRNA)
Sample type RNA
Source name skin
Organism Homo sapiens
Characteristics disease state: Psoriasis
tissue: skin
Treatment protocol 3 total skin samples from patients with psoriasis and 3 samples from healthy controls were analysed
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions and subsequent RNA cleanup and concentration was performed using the Rneasy MinElure Cleanup Kit (Qiagen, Venlo, Netherlands)
Label biotin
Label protocol 200ng of total RNA were amplified and labeled using the GeneChip® WT PLUS Reagent Kit (Catalog Number 902281, Affymetrix, Santa Clara, CA)
Hybridization protocol Affymetrix Human Transcriptome Array 2.0 ST arrays were washed, stained and scanned according to the protocol described in GeneChip® WT Terminal Labeling and Hybridization User Manual
Scan protocol Scanning was done on an Affymetrix GeneChip Scanner 3000 7G, software AGCC 3.1.1
Description N/A
Data processing The data were analyzed withTranscriptome Analysis Console (TAC) 4.0.1 using default analysis settings. Row data were log2-transformed and normalized by Robust multichip average (RMA) algorithm normalization.
Submission date Feb 14, 2020
Last update date Dec 08, 2020
Contact name Markus Jeitler
Organization name Medical University Vienna
Street address Lazarettgasse 14
City Vienna
ZIP/Postal code A-1090
Country Austria
Platform ID GPL17586
Series (2)
GSE145304 Combined miRNA-mRNA transcriptional profiling in the human skin [mRNA_PSOR]
GSE145305 Combined miRNA-mRNA transcriptional profiling in the human skin

Data table header descriptions
VALUE normalized RMA expression values

Data table
TC01000001.hg.1 6.67395
TC01000002.hg.1 6.430975
TC01000003.hg.1 5.849301
TC01000004.hg.1 6.95334
TC01000005.hg.1 8.728646
TC01000006.hg.1 14.66882
TC01000007.hg.1 12.92117
TC01000008.hg.1 8.24998
TC01000009.hg.1 5.465546
TC01000010.hg.1 6.443446
TC01000011.hg.1 8.005442
TC01000012.hg.1 8.069793
TC01000013.hg.1 18.68737
TC01000014.hg.1 7.491797
TC01000015.hg.1 6.065405
TC01000016.hg.1 6.853048
TC01000017.hg.1 5.928344
TC01000018.hg.1 7.848869
TC01000019.hg.1 6.925874
TC01000020.hg.1 5.910243

Total number of rows: 70523

Table truncated, full table size 1724 Kbytes.

Supplementary file Size Download File type/resource
GSM4314529_MJ_11272018_MIL_1_HTA-2_0_.CEL.gz 25.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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