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Sample GSM4314530 Query DataSets for GSM4314530
Status Public on Dec 07, 2020
Title Psoriasis_skin_sample_#2 (mRNA)
Sample type RNA
 
Source name skin
Organism Homo sapiens
Characteristics disease state: Psoriasis
tissue: skin
Treatment protocol 3 total skin samples from patients with psoriasis and 3 samples from healthy controls were analysed
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions and subsequent RNA cleanup and concentration was performed using the Rneasy MinElure Cleanup Kit (Qiagen, Venlo, Netherlands)
Label biotin
Label protocol 200ng of total RNA were amplified and labeled using the GeneChip® WT PLUS Reagent Kit (Catalog Number 902281, Affymetrix, Santa Clara, CA)
 
Hybridization protocol Affymetrix Human Transcriptome Array 2.0 ST arrays were washed, stained and scanned according to the protocol described in GeneChip® WT Terminal Labeling and Hybridization User Manual
Scan protocol Scanning was done on an Affymetrix GeneChip Scanner 3000 7G, software AGCC 3.1.1
Description N/A
Data processing The data were analyzed withTranscriptome Analysis Console (TAC) 4.0.1 using default analysis settings. Row data were log2-transformed and normalized by Robust multichip average (RMA) algorithm normalization.
 
Submission date Feb 14, 2020
Last update date Dec 08, 2020
Contact name Markus Jeitler
E-mail(s) markus.jeitler@meduniwien.ac.at
Organization name Medical University Vienna
Street address Lazarettgasse 14
City Vienna
ZIP/Postal code A-1090
Country Austria
 
Platform ID GPL17586
Series (2)
GSE145304 Combined miRNA-mRNA transcriptional profiling in the human skin [mRNA_PSOR]
GSE145305 Combined miRNA-mRNA transcriptional profiling in the human skin

Data table header descriptions
ID_REF
VALUE normalized RMA expression values

Data table
ID_REF VALUE
TC01000001.hg.1 6.581658
TC01000002.hg.1 6.201122
TC01000003.hg.1 5.979478
TC01000004.hg.1 6.346476
TC01000005.hg.1 8.347955
TC01000006.hg.1 14.10385
TC01000007.hg.1 12.15542
TC01000008.hg.1 8.359717
TC01000009.hg.1 5.715168
TC01000010.hg.1 5.987234
TC01000011.hg.1 7.768541
TC01000012.hg.1 7.910045
TC01000013.hg.1 18.72866
TC01000014.hg.1 6.567804
TC01000015.hg.1 5.912476
TC01000016.hg.1 6.435394
TC01000017.hg.1 6.024369
TC01000018.hg.1 7.648807
TC01000019.hg.1 6.743798
TC01000020.hg.1 5.891866

Total number of rows: 70523

Table truncated, full table size 1724 Kbytes.




Supplementary file Size Download File type/resource
GSM4314530_MJ_11272018_MIL_2_HTA-2_0_.CEL.gz 24.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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