GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM4314533 Query DataSets for GSM4314533
Status Public on Dec 07, 2020
Title Healthy_skin_sample_#2 (mRNA)
Sample type RNA
Source name skin
Organism Homo sapiens
Characteristics disease state: control
tissue: skin
Treatment protocol 3 total skin samples from patients with psoriasis and 3 samples from healthy controls were analysed
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions and subsequent RNA cleanup and concentration was performed using the Rneasy MinElure Cleanup Kit (Qiagen, Venlo, Netherlands)
Label biotin
Label protocol 200ng of total RNA were amplified and labeled using the GeneChip® WT PLUS Reagent Kit (Catalog Number 902281, Affymetrix, Santa Clara, CA)
Hybridization protocol Affymetrix Human Transcriptome Array 2.0 ST arrays were washed, stained and scanned according to the protocol described in GeneChip® WT Terminal Labeling and Hybridization User Manual
Scan protocol Scanning was done on an Affymetrix GeneChip Scanner 3000 7G, software AGCC 3.1.1
Description N/A
Data processing The data were analyzed withTranscriptome Analysis Console (TAC) 4.0.1 using default analysis settings. Row data were log2-transformed and normalized by Robust multichip average (RMA) algorithm normalization.
Submission date Feb 14, 2020
Last update date Dec 08, 2020
Contact name Markus Jeitler
Organization name Medical University Vienna
Street address Lazarettgasse 14
City Vienna
ZIP/Postal code A-1090
Country Austria
Platform ID GPL17586
Series (2)
GSE145304 Combined miRNA-mRNA transcriptional profiling in the human skin [mRNA_PSOR]
GSE145305 Combined miRNA-mRNA transcriptional profiling in the human skin

Data table header descriptions
VALUE normalized RMA expression values

Data table
TC01000001.hg.1 6.452363
TC01000002.hg.1 5.949885
TC01000003.hg.1 5.155978
TC01000004.hg.1 5.754909
TC01000005.hg.1 9.78161
TC01000006.hg.1 16.69177
TC01000007.hg.1 14.09442
TC01000008.hg.1 7.221695
TC01000009.hg.1 4.901422
TC01000010.hg.1 5.767409
TC01000011.hg.1 7.104845
TC01000012.hg.1 7.617731
TC01000013.hg.1 18.51766
TC01000014.hg.1 6.537617
TC01000015.hg.1 6.222144
TC01000016.hg.1 6.215054
TC01000017.hg.1 5.970277
TC01000018.hg.1 7.751791
TC01000019.hg.1 6.903936
TC01000020.hg.1 5.972651

Total number of rows: 70523

Table truncated, full table size 1724 Kbytes.

Supplementary file Size Download File type/resource
GSM4314533_MJ_11272018_MIL_5_HTA-2_0_.CEL.gz 24.0 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap