|
| Status |
Public on Dec 07, 2020 |
| Title |
Healthy_skin_sample_#2 (mRNA) |
| Sample type |
RNA |
| |
|
| Source name |
skin
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: control
tissue: skin
|
| Treatment protocol |
3 total skin samples from patients with psoriasis and 3 samples from healthy controls were analysed
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions and subsequent RNA cleanup and concentration was performed using the Rneasy MinElure Cleanup Kit (Qiagen, Venlo, Netherlands)
|
| Label |
biotin
|
| Label protocol |
200ng of total RNA were amplified and labeled using the GeneChip® WT PLUS Reagent Kit (Catalog Number 902281, Affymetrix, Santa Clara, CA)
|
| |
|
| Hybridization protocol |
Affymetrix Human Transcriptome Array 2.0 ST arrays were washed, stained and scanned according to the protocol described in GeneChip® WT Terminal Labeling and Hybridization User Manual
|
| Scan protocol |
Scanning was done on an Affymetrix GeneChip Scanner 3000 7G, software AGCC 3.1.1
|
| Description |
N/A
|
| Data processing |
The data were analyzed withTranscriptome Analysis Console (TAC) 4.0.1 using default analysis settings. Row data were log2-transformed and normalized by Robust multichip average (RMA) algorithm normalization.
|
| |
|
| Submission date |
Feb 14, 2020 |
| Last update date |
Dec 08, 2020 |
| Contact name |
Markus Jeitler |
| E-mail(s) |
markus.jeitler@meduniwien.ac.at
|
| Organization name |
Medical University Vienna
|
| Street address |
Lazarettgasse 14
|
| City |
Vienna |
| ZIP/Postal code |
A-1090 |
| Country |
Austria |
| |
|
| Platform ID |
GPL17586 |
| Series (2) |
| GSE145304 |
Combined miRNA-mRNA transcriptional profiling in the human skin [mRNA_PSOR] |
| GSE145305 |
Combined miRNA-mRNA transcriptional profiling in the human skin |
|
