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Sample GSM4318503 Query DataSets for GSM4318503
Status Public on Mar 01, 2021
Title day2 MCH-treat rep 2
Sample type SRA
 
Source name Cell culture
Organism Mus musculus
Characteristics strain: 3T3-L1 preadipocytes
Treatment protocol Insulin-induced differentiation and MCH treatment
Growth protocol Cell line standard culture protocol based on ATCC
Extracted molecule total RNA
Extraction protocol RNA extracted using Rneasy Mini Kit(Qiagen)
WaferGen PrepXTM mRNA Strand-Specific Library Preparation Protocol (WaferGen Biosystems)
Illumina mulitplex indexed single-end
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
 
Data processing alignment by STAR v2.5.2a
Reads count by HTSEQ v0.11.2
filtering out low-expressed genes
DESeq2 v1.24.0
Genome_build: mm10
Supplementary_files_format_and_content: reads count data, Excel .xlsx file format
 
Submission date Feb 18, 2020
Last update date Mar 01, 2021
Contact name Rongkun Shen
Organization name SUNY Brockport
Department Biology
Street address 350 New Campus Dr
City Brockport
State/province New York
ZIP/Postal code 14420-2997
Country USA
 
Platform ID GPL21493
Series (1)
GSE145452 Transcriptome Analysis of Ciliary-Dependent MCH Signaling in Differentiating 3T3-L1 Preadipocytes
Relations
BioSample SAMN14124653
SRA SRX7739456

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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