|
| Status |
Public on Feb 01, 2010 |
| Title |
melanoma short-term culture M010403 |
| Sample type |
RNA |
| |
|
| Source name |
melanoma short-term culture M010403
|
| Organism |
Homo sapiens |
| Characteristics |
melanoma type: patient-derived short-term culture M010403
|
| Treatment protocol |
cells were grown under standard conditions (no treatment)
|
| Growth protocol |
Cell lines and short-term cultures were grown as described in Lin et al, Cancer Research (2008), 68:664-673
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
cRNA were hybridized according to the standard Affymetrix protocol
|
| Scan protocol |
data were scanned according to standard protocols
|
| Description |
gene expression data, microarray-derived
|
| Data processing |
data were processed using the aroma.affymetrix package in Bioconductor. RMA (robust multichip average) was applied in combination with quantile normalization to generate gene centric expression values.
|
| |
|
| Submission date |
Jul 27, 2009 |
| Last update date |
Feb 04, 2010 |
| Contact name |
Michael Berger |
| E-mail(s) |
berger@broadinstitute.org
|
| Organization name |
The Broad Instititute
|
| Department |
Cancer Program
|
| Street address |
7 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL5175 |
| Series (2) |
| GSE17349 |
Expression data for melanoma short-term cultures and cell lines |
| GSE17593 |
Melanoma short-term cultures and cell lines: expression profiling and CNV analyses |
|
