|
Status |
Public on Feb 03, 2011 |
Title |
HeLa_sodium butyrate_1 |
Sample type |
RNA |
|
|
Source name |
HeLa 15 hrs NaB treatment
|
Organism |
Homo sapiens |
Characteristics |
cell line: HeLa agent: sodium butyrate
|
Treatment protocol |
15 hrs treatment with 5mM sodium butyrate
|
Growth protocol |
HeLa cells cultured in DMEM supplemented with 10% fetal calf serum, penicillin, and streptomycin.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA isolated with TRIzol
|
Label |
biotin
|
Label protocol |
Affymetrix GeneChip Whole Transcript (WT) Sense Target Labeling Assay (P/N 701880 Rev. 5)
|
|
|
Hybridization protocol |
Affymetrix GeneChip Whole Transcript (WT) Sense Target Labeling Assay (P/N 701880 Rev. 5)
|
Scan protocol |
Affymetrix GeneChip Expression Wash, Stain, and Scan User Manual (P/N 702731)
|
Description |
HeLa_sodium butyrate_1
|
Data processing |
Data were analysed using Partek Genomics Suite 6.4 software (RMA, quantile normalization). Only ‘core’ meta-probe sets were used to identify alternative splicing events.
|
|
|
Submission date |
Jul 29, 2009 |
Last update date |
Feb 03, 2011 |
Contact name |
David Stanek |
E-mail(s) |
stanek@img.cas.cz
|
Organization name |
Institute of Molecular Genetics
|
Street address |
Videnska 1083
|
City |
Prague |
ZIP/Postal code |
14220 |
Country |
Czech Republic |
|
|
Platform ID |
GPL5188 |
Series (1) |
GSE17397 |
Alternative splicing in HeLa cells after sodium butyrate treatment |
|