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Sample GSM434590 Query DataSets for GSM434590
Status Public on Feb 03, 2011
Title HeLa_sodium butyrate_1
Sample type RNA
 
Source name HeLa 15 hrs NaB treatment
Organism Homo sapiens
Characteristics cell line: HeLa
agent: sodium butyrate
Treatment protocol 15 hrs treatment with 5mM sodium butyrate
Growth protocol HeLa cells cultured in DMEM supplemented with 10% fetal calf serum, penicillin, and streptomycin.
Extracted molecule total RNA
Extraction protocol Total RNA isolated with TRIzol
Label biotin
Label protocol Affymetrix GeneChip Whole Transcript (WT) Sense Target Labeling Assay (P/N 701880 Rev. 5)
 
Hybridization protocol Affymetrix GeneChip Whole Transcript (WT) Sense Target Labeling Assay (P/N 701880 Rev. 5)
Scan protocol Affymetrix GeneChip Expression Wash, Stain, and Scan User Manual (P/N 702731)
Description HeLa_sodium butyrate_1
Data processing Data were analysed using Partek Genomics Suite 6.4 software (RMA, quantile normalization). Only ‘core’ meta-probe sets were used to identify alternative splicing events.
 
Submission date Jul 29, 2009
Last update date Feb 03, 2011
Contact name David Stanek
E-mail(s) stanek@img.cas.cz
Organization name Institute of Molecular Genetics
Street address Videnska 1083
City Prague
ZIP/Postal code 14220
Country Czech Republic
 
Platform ID GPL5188
Series (1)
GSE17397 Alternative splicing in HeLa cells after sodium butyrate treatment

Data table header descriptions
ID_REF
VALUE RMA sigmal estimates from Partek Genomics Suite 6.4 software

Data table
ID_REF VALUE
2315588 6.16271
2315589 6.94932
2315591 5.20635
2315594 6.07231
2315595 4.50660
2315596 4.73781
2315598 3.42050
2315602 4.67182
2315603 6.14694
2315604 5.59799
2315607 4.29668
2315638 7.89676
2315639 6.86813
2315640 8.01666
2315641 6.70160
2315642 7.50600
2315643 8.22032
2315644 6.56168
2315645 7.23792
2315690 5.32944

Total number of rows: 228871

Table truncated, full table size 3581 Kbytes.




Supplementary file Size Download File type/resource
GSM434590.CEL.gz 37.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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