|
| Status |
Public on Jun 21, 2010 |
| Title |
CB - 4 - mAdbID:98292 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
EBV-infected lymphocytes (reference)
|
| Organisms |
Homo sapiens; human gammaherpesvirus 4 |
| Characteristics |
cell type: Lymphoblastoid cells transfected with Epstein-Barr virus (EBV)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol Extraction Protocol
Other: Cells were lysed with TRIzol reagent (Invitrogen, Carlsbad, CA) and total RNA was isolated according to the manufacturers instructions. RNA integrity was assessed by the presence of 28S and 18S bands using Agilent bioanalyzer and quantified using nanodrop with A260/A280 ratios > 2.0 after dilution in DEPC water.
|
| Label |
Cy3
|
| Label protocol |
Hy3 Labeling Protocol
Other: 2 µg total RNA was directly labeled with miRCURY™ LNA Array Power Labeling Kit (Exiqon, Woburn, MA) according to manufacture’s procedure.
|
| |
|
| Channel 2 |
| Source name |
umbilical cord erythroid
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: umbilical cord blood
developmental stage: fetus
cell type: erythroid cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol Extraction Protocol
Other: Cells were lysed with TRIzol reagent (Invitrogen, Carlsbad, CA) and total RNA was isolated according to the manufacturers instructions. RNA integrity was assessed by the presence of 28S and 18S bands using Agilent bioanalyzer and quantified using nanodrop with A260/A280 ratios > 2.0 after dilution in DEPC water.
|
| Label |
Cy5
|
| Label protocol |
Hy5 Labeling Protocol
Other: 2 µg total RNA was directly labeled with miRCURY™ LNA Array Power Labeling Kit (Exiqon, Woburn, MA) according to manufacture’s procedure.
|
| |
|
| |
| Hybridization protocol |
Sample Hybridization Protocol
Other: The samples and the reference were co-hybridized to the miRNA array at room temperature over night. Both the processed cDNA and the miRNA array slides were scanned by GenePix scanner Pro 4.0 (Axon, Sunnyvale, CA, USA).
|
| Scan protocol |
Creator: GenePix Pro 4.0.1.23
Scanner: GenePix 4000B [84945]
ScanPower: 10;; 33
LaserPower: 3.17;; 3.44
Temperature: 32.8
|
| Description |
mAdb experiment ID: 98292
|
| Data processing |
miRNA Data Processing
Calculation Method: The raw signal intensities were imported into BRRArray Tools. signal intensity were background-intensity subtracted. Data are further normalized by median value over entire array.
|
| |
|
| Submission date |
Jul 29, 2009 |
| Last update date |
Jun 21, 2010 |
| Contact name |
Francesco Maria Marincola |
| E-mail(s) |
fmarincola@sidra.org
|
| Phone |
301-793-8210
|
| Organization name |
Sidra Medical and Research Center
|
| Street address |
Al Nasr Tower, AL Corniche Street, PO Box 26999
|
| City |
Doha |
| ZIP/Postal code |
PO Box 26999 |
| Country |
Qatar |
| |
|
| Platform ID |
GPL8949 |
| Series (1) |
| GSE17405 |
Let-7 microRNAs are developmentally regulated in circulating human erythroid cells |
|
