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Sample GSM434883 Query DataSets for GSM434883
Status Public on Jul 31, 2009
Title Stmn1-WT v Stmn1-null Array 1
Sample type RNA
 
Channel 1
Source name Total RNA extraction from tissue culture of stmn1 +/+ embryonic fibroblasts
Organism Mus musculus
Characteristics strain: C57BL/6
genotype: wild type
cell type: embryonic fibroblasts
Extracted molecule total RNA
Extraction protocol Used Trizol Extration from cultured cells followed by BioChip quality check, DNase treatment, quantification of RNA.
Label Cy5
Label protocol Followed Agilent protocol for linear amplification of cDNA sample labeling.
 
Channel 2
Source name Total RNA extraction from tissue culture of stmn1 -/- embryonic fibroblasts
Organism Mus musculus
Characteristics strain: C57BL/6
genotype: stmn1 -/-
cell type: embryonic fibroblasts
Extracted molecule total RNA
Extraction protocol Used Trizol Extration from cultured cells followed by BioChip quality check, DNase treatment, quantification of RNA.
Label Cy3
Label protocol Followed Agilent protocol for linear amplification of cDNA sample labeling.
 
 
Hybridization protocol default Agilent protocol
Scan protocol default Agilent protocol
Description n/a
Data processing default Agilent protocol
 
Submission date Jul 30, 2009
Last update date Jul 30, 2009
Contact name Danielle N. Ringhoff
E-mail(s) dnr204@lehigh.edu
Organization name Lehigh University
Department Biological Sciences
Lab Cassimeris
Street address 111 Research Drive
City Bethlehem
State/province PA
ZIP/Postal code 18015
Country USA
 
Platform ID GPL4134
Series (1)
GSE17432 Murine Embryonic Fibroblasts: Wild-type Stmn1 versus null Stmn1 Cells

Data table header descriptions
ID_REF
VALUE -[INV_VALUE]: normalized log10 ratio Cy5/Cy3
INV_VALUE normalized log10 ratio Cy5/Cy3

Data table
ID_REF VALUE INV_VALUE
1 0.126716 -1.267157792e-001
2 0.000000000e+000 0.000000000e+000
3 0.0231072 -2.310724293e-002
4 0.000000000e+000 0.000000000e+000
5 0.000000000e+000 0.000000000e+000
6 0.000000000e+000 0.000000000e+000
7 0.000000000e+000 0.000000000e+000
8 0.000000000e+000 0.000000000e+000
9 0.000000000e+000 0.000000000e+000
10 0.000000000e+000 0.000000000e+000
11 0.000000000e+000 0.000000000e+000
12 0.000000000e+000 0.000000000e+000
13 -0.538969 5.389694765e-001
14 0.190345 -1.903453850e-001
15 0.379697 -3.796974453e-001
16 0.172901 -1.729011700e-001
18 0.330818 -3.308181361e-001
19 -0.0271198 2.711983311e-002
20 0.148585 -1.485854945e-001
21 0.000000000e+000 0.000000000e+000

Total number of rows: 45018

Table truncated, full table size 1524 Kbytes.




Supplementary file Size Download File type/resource
GSM434883_WTvKO-01.txt.gz 15.3 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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