|
| Status |
Public on Sep 03, 2009 |
| Title |
std1 deletion strain vs. WT in Gal. rep 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
WT diploid strain BYXXX
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: BYXXX
|
| Growth protocol |
Each strain was grown to A600 = 1-1.2 in synthetic complete media + 2% galactose, incubated 2h and RNA was extracted using the hot phenol method.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cell pellets by hot phenol extraction.
|
| Label |
Cy3
|
| Label protocol |
Genisphere Array 350 Expression Array Detection Kit
|
| |
|
| Channel 2 |
| Source name |
Homozygous, std1 deletion strain
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: std1 deletion
|
| Growth protocol |
Each strain was grown to A600 = 1-1.2 in synthetic complete media + 2% galactose, incubated 2h and RNA was extracted using the hot phenol method.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cell pellets by hot phenol extraction.
|
| Label |
Cy5
|
| Label protocol |
Genisphere Array 350 Expression Array Detection Kit
|
| |
|
| |
| Hybridization protocol |
The Microarray Core facility at Washington University performed hybridization.
|
| Scan protocol |
Scanning was performed using a Scan Express HT scanner (Perkin Elmer).
|
| Description |
none
|
| Data processing |
The LOWESS algorithm was used for normalization of each array.
|
| |
|
| Submission date |
Jul 30, 2009 |
| Last update date |
Sep 03, 2009 |
| Contact name |
Jeffrey Sabina |
| Organization name |
Washington University in Saint Louis
|
| Department |
Genetics
|
| Lab |
M. Johnston
|
| Street address |
4444 Forest Park Blvd.
|
| City |
Saint Louis |
| ZIP/Postal code |
63108 |
| Country |
USA |
| |
|
| Platform ID |
GPL7882 |
| Series (1) |
| GSE17437 |
Deletion mutants mth1, std1, and mth1+std1 v. wild type yeast grown in galactose |
|
