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Sample GSM435244 Query DataSets for GSM435244
Status Public on Feb 02, 2013
Title H17M_rep2_Array4
Sample type RNA
 
Source name whole body, 7-day-old adult, male
Organism Drosophila pseudoobscura x Drosophila persimilis
Characteristics tissue: whole body
gender: Male
age: 7-day-old
Growth protocol Flies were reared on cornmeal/yeast medium and maintained at 20°C with a 12:12-h light/dark cycle.
Extracted molecule total RNA
Extraction protocol Flies were instantly frozen in liquid nitrogen and stored in TRIZOL (Invitrogen) at -80°C. RNA was isolated using TRIZOL from at least 100 individuals. RNA quantity and quality were separately measured on a NanoDrop spectrophotometer (Thermo Scientific) and a Bioanalyzer 2100 (Agilent Technologies). High-quality samples (260/280>1.85; 260/230>1.7) further purified using the RNeasy kit (Qiagen).
Label Cy3
Label protocol Fluorescent cRNA was synthesized from 200 ng of total RNA using Agilent’s low-RNA input fluorescent linear amplification kit following manufacturer's protocols (Agilent Technologies). Labelled RNAs were cleaned with RNeasy columns (Qiagen), and cRNA yields were quantified on a NanoDrop spectrophotometer. Two separate labeling reactions per sample were pooled and hybridized to the arrays following Agilent’s protocols.
 
Hybridization protocol Agilent’s protocols
Scan protocol Agilent’s protocols
Description Hybrids of Drosophila pseudoobscura (maternal) and Drosophila persimilis (paternal)
Data processing Performed in HDBStat: data is normalized by flagging out bad spots, filtering unexpressed genes (average raw intensity across all the samples <50), negative and positive Agilent controls, and then normalized with Chip Mean and log2 transformation.
 
Submission date Jul 31, 2009
Last update date Feb 02, 2013
Contact name Carlos Alberto Machado
E-mail(s) zjiang@umd.edu
Organization name University of Maryland
Department department of biology
Street address 1210 BPS
City college park
State/province MD
ZIP/Postal code 20742
Country USA
 
Platform ID GPL8884
Series (1)
GSE17452 Aberrant gene expression in Drosophila hybrids supporting faster-male evolution

Data table header descriptions
ID_REF
VALUE normalized signal

Data table
ID_REF VALUE
GA10247_4 0.714067325
GA10247_1 1.218105994
GA10310_1PS 3.514498228
GA10310_1PER 3.755512918
GA10395_3 0.743999421
GA10395_4 0.887117323
GA10397_3 0.361496731
GA10397_1 0.388677834
GA10397_2 0.328604584
GA10397_4 0.40356006
GA10423_1PER 0.418290333
GA10423_1PS 0.423167191
GA10493_1 0.916466614
GA10493_3 0.827300458
GA10493_4 0.959400543
GA10493_2 0.858599352
GA10495_4 0.653933633
GA10495_1 1.039711525
GA10495_2 0.761048859
GA10497_4 0.362512624

Total number of rows: 25699

Table truncated, full table size 580 Kbytes.




Supplementary file Size Download File type/resource
GSM435244.gpr.gz 1.8 Mb (ftp)(http) GPR
Processed data included within Sample table

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