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Sample GSM435250 Query DataSets for GSM435250
Status Public on Feb 02, 2013
Title H27M_rep2_Array2
Sample type RNA
 
Source name whole body, 7-day-old adult, male
Organism Drosophila persimilis x Drosophila pseudoobscura
Characteristics tissue: whole body
gender: Male
age: 7-day-old
Growth protocol Flies were reared on cornmeal/yeast medium and maintained at 20°C with a 12:12-h light/dark cycle.
Extracted molecule total RNA
Extraction protocol Flies were instantly frozen in liquid nitrogen and stored in TRIZOL (Invitrogen) at -80°C. RNA was isolated using TRIZOL from at least 100 individuals. RNA quantity and quality were separately measured on a NanoDrop spectrophotometer (Thermo Scientific) and a Bioanalyzer 2100 (Agilent Technologies). High-quality samples (260/280>1.85; 260/230>1.7) further purified using the RNeasy kit (Qiagen).
Label Cy3
Label protocol Fluorescent cRNA was synthesized from 200 ng of total RNA using Agilent’s low-RNA input fluorescent linear amplification kit following manufacturer's protocols (Agilent Technologies). Labelled RNAs were cleaned with RNeasy columns (Qiagen), and cRNA yields were quantified on a NanoDrop spectrophotometer. Two separate labeling reactions per sample were pooled and hybridized to the arrays following Agilent’s protocols.
 
Hybridization protocol Agilent’s protocols
Scan protocol Agilent’s protocols
Description Hybrids of Drosophila pseudoobscura (paternal) and Drosophila persimilis (maternal)
Data processing Performed in HDBStat: data is normalized by flagging out bad spots, filtering unexpressed genes (average raw intensity across all the samples <50), negative and positive Agilent controls, and then normalized with Chip Mean and log2 transformation.
 
Submission date Jul 31, 2009
Last update date Feb 02, 2013
Contact name Carlos Alberto Machado
E-mail(s) zjiang@umd.edu
Organization name University of Maryland
Department department of biology
Street address 1210 BPS
City college park
State/province MD
ZIP/Postal code 20742
Country USA
 
Platform ID GPL8884
Series (1)
GSE17452 Aberrant gene expression in Drosophila hybrids supporting faster-male evolution

Data table header descriptions
ID_REF
VALUE normalized signal

Data table
ID_REF VALUE
GA10247_4 0.63479016
GA10247_1 1.157413658
GA10310_1PS 3.836262268
GA10310_1PER 4.003757683
GA10395_3 0.352246316
GA10395_4 0.386751694
GA10397_3 0.347874533
GA10397_1 0.371757035
GA10397_2 0.329145227
GA10397_4 0.382483385
GA10423_1PER 0.474590098
GA10423_1PS 0.48855814
GA10493_1 0.937933565
GA10493_3 0.83392448
GA10493_4 0.959585145
GA10493_2 0.894380144
GA10495_4 0.656163716
GA10495_1 1.051843336
GA10495_2 0.979504258
GA10497_4 0.31913039

Total number of rows: 25699

Table truncated, full table size 579 Kbytes.




Supplementary file Size Download File type/resource
GSM435250.gpr.gz 1.8 Mb (ftp)(http) GPR
Processed data included within Sample table

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