|
| Status |
Public on Oct 15, 2009 |
| Title |
whole body 3 |
| Sample type |
RNA |
| |
|
| Source name |
E16.5 Mouse
|
| Organism |
Mus musculus |
| Characteristics |
tissue: whole body
developmental stage: embryo
age: E16.5
|
| Treatment protocol |
Laser capture microdissection of fresh frozen tissue
|
| Growth protocol |
SAC pregnant females at noon 16.5 days after detection of the copulation plug
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Molecular Devices Pico-Pure Isolation Kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized on [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Standard Affymetrix Protocol
|
| Description |
Gene expression data E16.5 TMJ
|
| Data processing |
Data was analyzed using Limma on Bioconductor for R
|
| |
|
| Submission date |
Aug 03, 2009 |
| Last update date |
Aug 11, 2009 |
| Contact name |
Daniel Joseph OConnell |
| E-mail(s) |
daniel.j.oconnell@gmail.com
|
| Phone |
617 525 4711
|
| Fax |
617 525 4751
|
| Organization name |
Harvard Medical School
|
| Department |
Genetics
|
| Lab |
Richard Maas
|
| Street address |
77 Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE17473 |
RNA expression data of embryonic E16.5 mouse temporomandibular joint (TMJ) |
|
