|
| Status |
Public on Jul 24, 2001 |
| Title |
fem-3(q23) (red, channel 2) vs. fem-1(hc17) (green, channel 1) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
fem-1(hc17) (green)
|
| Organism |
Caenorhabditis elegans |
| Extracted molecule |
total RNA |
| |
|
| Channel 2 |
| Source name |
fem-3(q23) (red)
|
| Organism |
Caenorhabditis elegans |
| Extracted molecule |
total RNA |
| |
|
| |
| Description |
Category: germline
Subcategory: sperm/oocyte
Experimental details: fem-1(hc17) is a temperature sensitive loss-of-function mutation which, at 25 degrees Celsius, produces only oocytes and no sperm. Female soma and germline. fem-3(q23) is a temperature sensitive gain-of-function mutation which, at 25 degrees C, continually produces spermatids. Soma is female, germline is male. Both are fertile at 15 degrees. Growth conditions: Synchronized populations of worms were grown at 15C on15cm enriched peptone agar plates seed with E.Coli. Worms were purified by pouring over a Nytex filter. Total RNA was isolated using the glass bead extraction protocol. Poly(A)+ RNA was isolated using the Invitrogen FastTrack 2.0 kit.
Scanning Software: GenePix
Software version: 2.0.1.15
Scanning parameters:
Lot batch = rosetta_10934 (expt 1)
|
| |
|
| Submission date |
Jul 24, 2001 |
| Last update date |
May 27, 2005 |
| Organization |
Stanford Microarray Database (SMD) |
| E-mail(s) |
array@genome.stanford.edu
|
| Phone |
650-498-6012
|
| URL |
http://genome-www5.stanford.edu/
|
| Department |
Stanford University, School of Medicine
|
| Street address |
300 Pasteur Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL14 |
| Series (1) |
| GSE5 |
Global profile of germline gene expression in C. elegans |
|
| Data table header descriptions |
| ID_REF |
|
| CH1B_MEAN |
mean channel 1 background |
| CH1B_MEDIAN |
median channel 1 background |
| CH1D_MEAN |
difference between CH1I_MEAN and CH1B_MEAN |
| CH2I_MEAN |
mean channel 2 signal |
| CH2D_MEAN |
difference of CH2I_MEAN and CH2B_MEAN |
| CH2B_MEAN |
mean of channel 2 background |
| CH2B_MEDIAN |
median of channel 2 background |
| CH2BN_MEDIAN |
normalized CH2B_MEDIAN |
| CH2DN_MEAN |
normalized CH2D_MEAN |
| CH2IN_MEAN |
normalized CH2I_MEAN |
| CORR |
simple correlation coefficient of channel 1 and channel 2 pixels |
| FLAG |
user defined flag (default=0) |
| VALUE |
aka LOG_RAT2N_MEAN, or log2 of ratio of CH2DN_MEAN and CH1DN_MEAN |
| PIX_RAT2_MEDIAN |
|
| PERGTBCH1I_1SD |
standard deviation of fraction of pixels greater than CH1B_MEAN |
| PERGTBCH2I_1SD |
standard deviation of fraction of pixels greater than CH2B_MEAN |
| RAT1_MEAN |
ratio of CH1D_MEAN to CH2D_MEAN |
| RAT1N_MEAN |
ratio of CH1DN_MEAN to CH2DN_MEAN |
| RAT2_MEAN |
ratio of CH2D_MEAN to CH1D_MEAN |
| RAT2N_MEAN |
ratio of CH2DN_MEAN and CH1DN_MEAN |
| REGR |
slope of the simple regression line of channel 2 to channel 1 pixels |
| TOT_BPIX |
total number of background pixels |
| TOT_SPIX |
total number of signal (spot) pixels |
| TOP |
vertical, short-axis spot ellipse minimum pixel coordinate |
| BOT |
vertical, short-axis spot ellipse maximum pixel coordinate |
| LEFT |
horizontal, long-axis spot ellipse minimum pixel coordinate |
| RIGHT |
horizontal, long-axis spot ellipse maximum pixel coordinate |
