|
| Status |
Public on Sep 04, 2009 |
| Title |
C57BL/6 uninfected macrophages |
| Sample type |
RNA |
| |
|
| Source name |
bone marrow-derived macrophages
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
l. major infection: uninfected
gender: female
tissue: Bone marrow
cell type: macrophage
age: 6-8 weeks
|
| Treatment protocol |
After maturation of macrophage precursors, the cells were washed and incubated for 4 hours with or without L. major promoastigotes. The cells were then washed free of unbound parasites and incubated for 24hours before harvesting for RNA preparation.
|
| Growth protocol |
Bone marrow-derived macrophages from 6 mice each of C57BL/6, BALB/c, C.B6-(lmr1, lmr2) and B6.c-(lmr1, lmr2) were cultured for 3 days in the presence of FCS and L-cell conditioned media. After 2 days, adherent cells were discarded. This served to removed any fibroblasts present in the culture. The remaining cells were cultured for a further 4 days allowing non-adherent macrophage precursors to mature and adhere to the culture dish.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared by using RNeasy (Qiagen, Valencia, CA).
|
| Label |
biotin
|
| Label protocol |
Samples were labelled using the Enzo BioArray HighYield RNA Transcript Labeling kit – Affymetrix, following the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Samples were hybridised using Affymetrix protocols.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
B6.U
|
| Data processing |
The data were background corrected, normalized and summarized with the Bioconductor package gcrma. Full (fast=FALSE) parameter estimation was used.
|
| |
|
| Submission date |
Aug 06, 2009 |
| Last update date |
Aug 06, 2009 |
| Contact name |
Gordon K Smyth |
| E-mail(s) |
smyth@wehi.edu.au
|
| Phone |
(+61 3) 9345 2326
|
| Fax |
(+61 3) 9347 0852
|
| URL |
http://www.wehi.edu.au
|
| Organization name |
Walter and Eliza Hall Institute of Medical Research
|
| Department |
Bioinformatics
|
| Lab |
Smyth
|
| Street address |
1G Royal Pde
|
| City |
Parkville |
| State/province |
Vic |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE17550 |
Gene expression profiles of in vitro-infected (Leishmania major) and uninfected mouse bone marrow-derived macrophages |
|
