|
Status |
Public on Aug 31, 2009 |
Title |
INPUT ZT7, replica 1 |
Sample type |
RNA |
|
|
Source name |
fly head
|
Organism |
Drosophila melanogaster |
Characteristics |
time point: ZT7 replicate: 1 tissue: head developmental stage: adult
|
Growth protocol |
Flies were grown under standard conditions in 12hs light and 12hs dark cycles
|
Extracted molecule |
total RNA |
Extraction protocol |
Heads were collected and RNA extracted using TRIZOL
|
Label |
biotin
|
Label protocol |
RNA was utilized to prepare cDNA and cRNA using the Affymetrix IVT labeling Kit
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 18 hrs at 45C on GeneChip Drosophila Genome 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned with the Affymetrix GeneArray Scanner G7
|
Description |
INP sample, ZT7
|
Data processing |
The data were processed with GCRMA algorithm in the Bioconductor with default settings
|
|
|
Submission date |
Aug 13, 2009 |
Last update date |
Aug 28, 2018 |
Contact name |
Ken Sugino |
Organization name |
Janelia Research Campus
|
Lab |
NeuroSeq
|
Street address |
19700 Helix Dr
|
City |
Ashburn |
State/province |
VA |
ZIP/Postal code |
20147 |
Country |
USA |
|
|
Platform ID |
GPL1322 |
Series (2) |
GSE17627 |
AGO 1 IMMUNOPRECIPITATION MICROARRAYS |
GSE17629 |
Circadian analysis of miRNAs and their targets |
|
Relations |
Reanalyzed by |
GSE119084 |