|
| Status |
Public on Mar 12, 2020 |
| Title |
QM84C20 |
| Sample type |
RNA |
| |
|
| Source name |
0.3 M NaCl eluted Exo
|
| Organism |
Mus musculus |
| Characteristics |
sample type: CTL culture supernatant
|
| Treatment protocol |
Murine CTL culture supernatant was subjected to DEAE column chromatograpy after concentration of deproteinization of 750 kDa MWCO ultrafiltration.
|
| Growth protocol |
Murine CTL was cultured for 7days, and culture supernatant was collected.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted, and amplified with Ambion Amino Allyl aRNA kit.
|
| Label |
Cy5
|
| Label protocol |
Amplified aRNA was labeled with Amersham Cy5 mono-reactive dye (GE #PA25001)
|
| |
|
| Hybridization protocol |
Hybridization was performed with hybridyzation chamber (Takara Bio #TX711
|
| Scan protocol |
3D-Gene Scanner 3000 (Toray) was used in this study.
|
| Data processing |
After the image was acquired with the optimal PMT value (laser-100%), it was digitized with digitizing software.
|
| |
|
| Submission date |
Mar 11, 2020 |
| Last update date |
Mar 12, 2020 |
| Contact name |
Naohiro Seo |
| E-mail(s) |
seo-naohiro@clin.medic.mie-u.ac.jp
|
| Organization name |
Mie University Graduate School of Medicine
|
| Department |
Immuno-Gene Therpy
|
| Street address |
2-174 Edobashi
|
| City |
Tsu |
| State/province |
Mie |
| ZIP/Postal code |
514-8507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL22438 |
| Series (1) |
| GSE146815 |
mRNA microarray analysis of murine CTL EVs separated by ion exchange method [mRNA] |
|
